anti-Human SH2D1B Antibody | anti-SH2D1B antibody

SH2D1B Antibody

Cat. Num. AAA855997

• Gene Names: SH2D1B; EAT2
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: SH2D1B; SH2D1B Antibody; SH2 domain-containing protein 1B EWS/FLI1-activated transcript 2; EAT-2 SH2D1B; anti-SH2D1B antibody

• Reactivity: Human
• Specificity: SH2D1B antibody detects endogenous levels of total SH2D1B protein.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 132

• Applicable Applications for anti-SH2D1B antibody: Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:40000
• Immunogen: The antiserum was produced against synthesized peptide derived from internal of human SH2D1B.
• Preparation and Storage: Store at-20 degree C for 1 year.

Immunohistochemistry

(Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using SH2D1B antibody.)

Product Categories/Family for anti-SH2D1B antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

Morra M., EMBO J. 20:5840-5852(2001). Colonna M.; Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases. Tangye S.G., Eur. J. Immunol. 32:1640-1649(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 54792745
• NCBI GeneID: 117157
• NCBI Accession #: NP_444512.2
• NCBI GenBank Nucleotide #: NM_053282.4
• UniProt Accession #: O14796; Q5T0L1; Q8NI18; Q969K9; B2RBN6
• Molecular Weight: 9,024 Da
• NCBI Official Full Name: SH2 domain-containing protein 1B
• NCBI Official Synonym Full Names: SH2 domain containing 1B
• NCBI Official Symbol: SH2D1B
• NCBI Official Synonym Symbols: EAT2
• NCBI Protein Information: SH2 domain-containing protein 1B
• UniProt Protein Name: SH2 domain-containing protein 1B
• Protein Family: SH2 domain-containing protein
• UniProt Gene Name: SH2D1B
• UniProt Synonym Gene Names: EAT2; EAT-2

NCBI Description

By binding phosphotyrosines through its free SRC (MIM 190090) homology-2 (SH2) domain, EAT2 regulates signal transduction through receptors expressed on the surface of antigen-presenting cells (Morra et al., 2001 [PubMed 11689425]).[supplied by OMIM, Mar 2008]

Uniprot Description

Cytoplasmic adapter regulating receptors of the signaling lymphocytic activation molecule (SLAM) family such as CD84, SLAMF1, LY9 and CD244 (PubMed:11689425). In SLAM signaling seems to cooperate with SH2D1A/SAP. Plays a role in regulation of effector functions of natural killer (NK) cells by controlling signal transduction through CD244/2B4 without effecting its tyrosine phosphorylation; downstream signaling involves PLCG1 and ERK activation (PubMed:24687958). Activation of SLAMF7-mediated NK cell function does not effect receptor tyrosine phosphorylation but distal signaling (). In the context of NK cell-mediated cytotoxicity does not enhance conjugate formation with target cells but stimulates polarization of the microtubule-organizing center and cytotoxic granules toward the NK cell synapse (PubMed:24687958). Negatively regulates CD40-induced cytokine production in dendritic cells downstream of SLAM family receptors probably by inducing activation of the PI3K pathway to inhibit p38 MAPK and JNK activation ().