Rabbit AP2S1 Polyclonal Antibody | anti-AP2S1 antibody

AP2S1 Rabbit pAb

Cat. Num. AAA8548187

• Gene Names: AP2S1; AP17; FBH3; HHC3; FBHOk; CLAPS2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified
• Synonyms: AP2S1; Polyclonal Antibody; AP2S1 Rabbit pAb; AP17; FBH3; HHC3; FBHOk; CLAPS2; anti-AP2S1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purified
• Form/Format: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
• Sequence Length: 142

• Applicable Applications for anti-AP2S1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-142 of human AP2S1 (NP_004060.2).
• Cellular Localization: Cell Membrane, Coated Pit, Membrane
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Related Product Information for anti-AP2S1 antibody

Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein Transport via Transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif. May also play a role in extracellular calcium homeostasis.

NCBI and Uniprot Product Information

• NCBI GI #: 70906430
• NCBI GeneID: 1175
• NCBI Accession #: NP_004060.2
• NCBI GenBank Nucleotide #: NP_004060.2
• UniProt Accession #: P53680
• Molecular Weight: 17kDa
• NCBI Official Full Name: AP-2 complex subunit sigma isoform AP17
• NCBI Official Synonym Full Names: adaptor related protein complex 2 subunit sigma 1
• NCBI Official Symbol: AP2S1
• NCBI Official Synonym Symbols: AP17; FBH3; HHC3; FBHOk; CLAPS2
• NCBI Protein Information: AP-2 complex subunit sigma
• UniProt Protein Name: AP-2 complex subunit sigma
• Protein Family: AP-2 complex
• UniProt Gene Name: AP2S1
• UniProt Synonym Gene Names: AP17; CLAPS2
• UniProt Entry Name: AP2S1_HUMAN

NCBI Description

One of two major clathrin-associated adaptor complexes, AP-2, is a heterotetramer which is associated with the plasma membrane. This complex is composed of two large chains, a medium chain, and a small chain. This gene encodes the small chain of this complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Uniprot Description

AP2S1: Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein Transport via Transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin- coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif. Belongs to the adaptor complexes small subunit family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: plasma membrane; AP-2 adaptor complex; cytosol

Molecular Function: transporter activity; protein transporter activity

Biological Process: negative regulation of epidermal growth factor receptor signaling pathway; epidermal growth factor receptor signaling pathway; intracellular protein transport; axon guidance; synaptic transmission; clathrin cage assembly; viral reproduction; nerve growth factor receptor signaling pathway; regulation of endocytosis; ephrin receptor signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class II; regulation of defense response to virus by virus

Disease: Hypocalciuric Hypercalcemia, Familial, Type Iii