anti-Human iNOS Antibody | anti-iNOS antibody

iNOS Antibody

Cat. Num. AAA854129

• Gene Names: NOS2; NOS; INOS; NOS2A; HEP-NOS
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: iNOS; iNOS Antibody; NITRIC OXIDE SYNTHASE; INDUCIBLE; MACROPHAGE; NOS2A; HEPATOCYTE; nitric oxide synthase 2A (inducible; hepatocytes); anti-iNOS antibody

• Reactivity: Human
• Specificity: iNOS antibody detects endogenous levels of total iNOS protein.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 1153

• Applicable Applications for anti-iNOS antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:20000
• Immunogen: The antiserum was produced against synthesized peptide derived from human iNOS.
• Preparation and Storage: Store at-20 degree C for 1 year

Immunohistochemistry (IHC)

(Peptide-+ Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using iNOS antibody.)

Western Blot (WB)

(Western blot analysis of extracts from NIH/3T3 cells, using iNOS antibody.)

Immunofluorescence (IF)

(Peptide-+ Immunofluorescence analysis of HepG2 cells, using iNOS antibody.)

References

Rachel J. Jones, Am J Physiol Heart Circ Physiol, Jun 2007; 292: H2634-H2642. Katrin Linker, Nucleic Acids Res., Aug 2005; 33: 4813-4827. Donna-Marie McCafferty, Donna-Marie McCafferty, Lian-Qun Qiu, J. Am. Soc. Nephrol., Aug 2004; 15: 2066-2078.

NCBI and Uniprot Product Information

• NCBI GI #: 24041029
• NCBI GeneID: 4843
• NCBI Accession #: NP_000616.3
• NCBI GenBank Nucleotide #: NM_000625.4
• UniProt Accession #: P35228; O60757; O94994; Q16263; Q16692; Q4TTS5; Q9UD42; A1L3U5; B7ZLY2
• Molecular Weight: 126,749 Da
• NCBI Official Full Name: nitric oxide synthase, inducible
• NCBI Official Synonym Full Names: nitric oxide synthase 2
• NCBI Official Symbol: NOS2
• NCBI Official Synonym Symbols: NOS; INOS; NOS2A; HEP-NOS
• NCBI Protein Information: nitric oxide synthase, inducible
• UniProt Protein Name: Nitric oxide synthase, inducible
• Protein Family: Inositol 3-kinase
• UniProt Gene Name: NOS2
• UniProt Synonym Gene Names: NOS2A; HEP-NOS; Inducible NOS; iNOS

NCBI Description

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]

Uniprot Description

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body (PubMed:7531687, PubMed:7544004). In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2 (). As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM (PubMed:25417112). Involved in inflammation, enhances the synthesis of proinflammatory mediators such as IL6 and IL8 (PubMed:19688109).