Immunohistochemistry (IHC)
(Peptide-+ Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using Gastrin antibody.)
anti-Human, Mouse Gastrin Antibody | anti-GAST antibody
Gastrin Antibody
Gene Names
GAST; GAS
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
Gastrin; Gastrin Antibody; Component II; GAS; gastrin precursor; anti-GAST antibody
Reactivity
Human, Mouse
Specificity
Gastrin antibody detects endogenous levels of total Gastrin protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence
CMDFGRRSAEDEN
Sequence Length
101
Applicable Applications for anti-GAST antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:10000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:10000
Immunogen
The antiserum was produced against synthesized peptide derived from human Gastrin.
Preparation and Storage
Store at-20 degree C for 1 year
Immunohistochemistry (IHC)
(Peptide-+ Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using Gastrin antibody.)
Immunohistochemistry (IHC)
(Peptide-+ Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using Gastrin antibody.)
Western Blot (WB)
(Western blot analysis of extracts from NIH/3T3 cells, using Gastrin antibody.)
Western Blot (WB)
(Western blot analysis of extracts from NIH/3T3 cells, using Gastrin antibody.)
Immunofluorescence (IF)
(Peptide-+ Immunofluorescence analysis of HeLa cells, using Gastrin antibody.)
Immunofluorescence (IF)
(Peptide-+ Immunofluorescence analysis of HeLa cells, using Gastrin antibody.)
References
Marlène Dufresne, Physiol Rev, Jul 2006; 86: 805-847. T. C. Wang, Tillotson, M. Chulak, S. J. Brand, and E. V. Schmidt D. H. Yu, Am J Physiol Gastrointest Liver Physiol, Dec 1987; 253: 793. Masato Komori, Am J Physiol Gastrointest Liver Physiol, Dec 2002; 283: 1368.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
11,394 Da
NCBI Official Full Name
gastrin preproprotein
NCBI Official Synonym Full Names
gastrin
NCBI Official Symbol
GAST
NCBI Official Synonym Symbols
GAS
NCBI Protein Information
gastrin
UniProt Protein Name
Gastrin
Protein Family
UniProt Gene Name
GAST
UniProt Synonym Gene Names
GAS; G52; G34; G17; G14; G6
NCBI Description
Gastrin is a hormone whose main function is to stimulate secretion of hydrochloric acid by the gastric mucosa, which results in gastrin formation inhibition. This hormone also acts as a mitogenic factor for gastrointestinal epithelial cells. Gastrin has two biologically active peptide forms, G34 and G17. [provided by RefSeq, Jul 2008]
Uniprot Description
Gastrin stimulates the stomach mucosa to produce and secrete hydrochloric acid and the pancreas to secrete its digestive enzymes. It also stimulates smooth muscle contraction and increases blood circulation and water secretion in the stomach and intestine.
Research Articles on GAST
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Product Notes
The GAST gast (Catalog #AAA854021) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Gastrin Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Gastrin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000. Researchers should empirically determine the suitability of the GAST gast for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: CMDFGRRSAE DEN. It is sometimes possible for the material contained within the vial of "Gastrin, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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