Rabbit anti-Human, Mouse LN28B Polyclonal Antibody | anti-LN28B antibody

LN28B Polyclonal Antibody

Cat. Num. AAA8531659

• Gene Names: LIN28B; CSDD2
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: LN28B; Polyclonal Antibody; LN28B Polyclonal Antibody; Protein lin-28 homolog B; Lin-28B; LIN28B; CSDD2; anti-LN28B antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
• Sequence Length: 250

• Applicable Applications for anti-LN28B antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500-2000; ELISA: 1:5000-20000
• Immunogen: Synthesized peptide derived from human protein. at AA range: 60-140
• Preparation and Storage: Store at-20 degree C for 1 year.

NCBI and Uniprot Product Information

• NCBI GI #: 51972214
• NCBI GeneID: 389421
• NCBI Accession #: NP_001004317.1
• NCBI GenBank Nucleotide #: NM_001004317.3
• UniProt Accession #: Q6ZN17; Q5TCM4; A1L165; B2RPN6
• Molecular Weight: 27
• NCBI Official Full Name: protein lin-28 homolog B
• NCBI Official Synonym Full Names: lin-28 homolog B
• NCBI Official Symbol: LIN28B
• NCBI Official Synonym Symbols: CSDD2
• NCBI Protein Information: protein lin-28 homolog B
• UniProt Protein Name: Protein lin-28 homolog B
• UniProt Gene Name: LIN28B
• UniProt Synonym Gene Names: CSDD2; Lin-28B

NCBI Description

The protein encoded by this gene belongs to the lin-28 family, which is characterized by the presence of a cold-shock domain and a pair of CCHC zinc finger domains. This gene is highly expressed in testis, fetal liver, placenta, and in primary human tumors and cancer cell lines. It is negatively regulated by microRNAs that target sites in the 3' UTR, and overexpression of this gene in primary tumors is linked to the repression of let-7 family of microRNAs and derepression of let-7 targets, which facilitates cellular transformation. [provided by RefSeq, Jun 2012]

Uniprot Description

Suppressor of microRNA (miRNA) biogenesis, including that of let-7 and possibly of miR107, miR-143 and miR-200c. Binds primary let-7 transcripts (pri-let-7), including pri-let-7g and pri-let-7a-1, and sequester them in the nucleolus, away from the microprocessor complex, hence preventing their processing into mature miRNA (PubMed:22118463). Does not act on pri-miR21 (PubMed:22118463). The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state of embryonic stem cells by preventing let-7-mediated differentiation. When overexpressed, recruits ZCCHC11/TUT4 uridylyltransferase to pre-let-7 transcripts, leading to their terminal uridylation and degradation (PubMed:19703396). This activity might not be relevant in vivo, as LIN28B-mediated inhibition of let-7 miRNA maturation appears to be ZCCHC11-independent (PubMed:22118463). Interaction with target pre-miRNAs occurs via an 5'-GGAG-3' motif in the pre-miRNA terminal loop. Mediates MYC-induced let-7 repression (). When overexpressed, isoform 1 stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.