Rabbit anti-Human, Mouse RAD1 Polyclonal Antibody | anti-RAD1 antibody

RAD1 Polyclonal Antibody

Cat. Num. AAA8530971

• Gene Names: RAD1; REC1; HRAD1
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: RAD1; Polyclonal Antibody; RAD1 Polyclonal Antibody; Cell cycle checkpoint protein RAD1; hRAD1; EC 3.1.11.2; DNA repair exonuclease rad1 homolog; Rad1-like DNA damage checkpoint protein; REC1; anti-RAD1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
• Sequence Length: 282

• Applicable Applications for anti-RAD1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500-2000; ELISA: 1:5000-20000
• Preparation and Storage: Store at-20 degree C for 1 year.

NCBI and Uniprot Product Information

• NCBI GI #: 4506385
• NCBI GeneID: 5810
• NCBI Accession #: NP_002844.1
• NCBI GenBank Nucleotide #: NM_002853.3
• UniProt Accession #: O60671; O75572; O95304; Q1W161; Q5KSM0; Q5KSM1; Q9UEP1
• Molecular Weight: 31
• NCBI Official Full Name: cell cycle checkpoint protein RAD1
• NCBI Official Synonym Full Names: RAD1 checkpoint DNA exonuclease
• NCBI Official Symbol: RAD1
• NCBI Official Synonym Symbols: REC1; HRAD1
• NCBI Protein Information: cell cycle checkpoint protein RAD1
• UniProt Protein Name: Cell cycle checkpoint protein RAD1
• Protein Family: DNA repair protein
• UniProt Gene Name: RAD1
• UniProt Synonym Gene Names: REC1; hRAD1

NCBI Description

This gene encodes a component of a heterotrimeric cell cycle checkpoint complex, known as the 9-1-1 complex, that is activated to stop cell cycle progression in response to DNA damage or incomplete DNA replication. The 9-1-1 complex is recruited by RAD17 to affected sites where it may attract specialized DNA polymerases and other DNA repair effectors. Alternatively spliced transcript variants of this gene have been described. [provided by RefSeq, Jan 2009]

Uniprot Description

Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair (PubMed:10846170, PubMed:10884395). The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex (PubMed:12578958). Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER) (PubMed:15871698). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates (PubMed:15314187, PubMed:15556996, PubMed:15871698). The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase (PubMed:21659603). Isoform 1 possesses 3'->5' double stranded DNA exonuclease activity (PubMed:9660799).