anti-Human ERCC3 Antibody | anti-ERCC3 antibody

ERCC3 Antibody

Cat. Num. AAA8526854

• Gene Names: ERCC3; XPB; BTF2; TTD2; GTF2H; RAD25; TFIIH
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Protein G-Sepharose affinity purified.
• Synonyms: ERCC3; ERCC3 Antibody; Mouse monoclonal IgG to ERCC3; anti-ERCC3 antibody

• Reactivity: Human
• Purity/Purification: Protein G-Sepharose affinity purified.
• Form/Format: Liquid
• Sequence Length: 782

• Applicable Applications for anti-ERCC3 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: ELISA: 1:5000-1:20000; WB: 1:100-1:2000
• Immunogen: Recombinant fragment of human ERCC3 (C-terminus).
• Preservative: 0.02% Sodium Azide and 50% glycerol, 1% BSA, PBS, pH7.4.
• Cellular Localization: Nucleus
• Preparation and Storage: Aliquot and store at-20 degree C. Avoid repeated freeze/thaw cycles.

Testing Data

(ERCC3 (C-terminus) antibody at 1/2000 dilution + recombinant ERCC3 protein with a GST tag. Predicted band size: 55KDa. Observed band size: 55KDa.)

Related Product Information for anti-ERCC3 antibody

ERCC3 is an ATP-dependent DNA helicase that functions in nucleotide excision repair and complements xeroderma pigmentosum group B mutations. It also is the 89 kDa subunit of basal transcription factor 2 (TFIIH) and thus functions in class II transcription.

NCBI and Uniprot Product Information

• NCBI GI #: 4557563
• NCBI GeneID: 2071
• NCBI Accession #: NP_000113.1
• NCBI GenBank Nucleotide #: NM_000122
• UniProt Accession #: P19447; Q53QM0
• Molecular Weight: 89,278 Da
• NCBI Official Full Name: general transcription and DNA repair factor IIH helicase subunit XPB isoform a
• NCBI Official Synonym Full Names: ERCC excision repair 3, TFIIH core complex helicase subunit
• NCBI Official Symbol: ERCC3
• NCBI Official Synonym Symbols: XPB; BTF2; TTD2; GTF2H; RAD25; TFIIH
• NCBI Protein Information: general transcription and DNA repair factor IIH helicase subunit XPB
• UniProt Protein Name: General transcription and DNA repair factor IIH helicase subunit XPB
• Protein Family: Probable DNA repair helicase
• UniProt Gene Name: ERCC3
• UniProt Synonym Gene Names: XPB; XPBC; TFIIH subunit XPB; BTF2 p89; TFIIH 89 kDa subunit; TFIIH p89

NCBI Description

This gene encodes an ATP-dependent DNA helicase that functions in nucleotide excision repair. The encoded protein is a subunit of basal transcription factor 2 (TFIIH) and, therefore, also functions in class II transcription. Mutations in this gene are associated with Xeroderma pigmentosum B, Cockayne's syndrome, and trichothiodystrophy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

Uniprot Description

ATP-dependent 3'-5' DNA helicase, component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The ATPase activity of XPB/ERCC3, but not its helicase activity, is required for DNA opening. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. The ATP-dependent helicase activity of XPB/ERCC3 is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.