anti-Human SGOL1 Antibody | anti-SGOL1 antibody

SGOL1 Antibody

Cat. Num. AAA8514053

• Gene Names: SGO1; SGO; CAID; SGOL1; NY-BR-85
• Reactivity: Human
• Applications: Western Blot
• Purity: Immunogen affinity purified
• Synonyms: SGOL1; SGOL1 Antibody; Shugoshin-like 1; hSgo1; Serologically defined breast cancer antigen NY-BR-85; SGO1; anti-SGOL1 antibody

• Reactivity: Human
• Specificity: SGOL1 Antibody detects endogenous levels of total SGOL1
• Purity/Purification: Immunogen affinity purified
• Form/Format: In phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 527

• Applicable Applications for anti-SGOL1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthesized peptide
• Preparation and Storage: Store at-20 degree C for 1 year

Western Blot (WB)

(Western blot analysis of extracts from HeLa cells, using SGOL1 antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 60302875
• NCBI GeneID: 151648
• NCBI Accession #: NP_001012409.1
• NCBI GenBank Nucleotide #: NM_001012409.3
• UniProt Accession #: Q5FBB7; Q588H5; Q5FBB4; Q5FBB5; Q5FBB6; Q5FBB8; Q8N579; Q8WVL0; Q9BVA8; Q9H275
• Molecular Weight: 64 KD
• NCBI Official Full Name: shugoshin 1 isoform 1
• NCBI Official Synonym Full Names: shugoshin 1
• NCBI Official Symbol: SGO1
• NCBI Official Synonym Symbols: SGO; CAID; SGOL1; NY-BR-85
• NCBI Protein Information: shugoshin 1
• UniProt Protein Name: Shugoshin 1
• UniProt Gene Name: SGO1

NCBI Description

The protein encoded by this gene is a member of the shugoshin family of proteins. This protein is thought to protect centromeric cohesin from cleavage during mitotic prophase by preventing phosphorylation of a cohesin subunit. Reduced expression of this gene leads to the premature loss of centromeric cohesion, mis-segregation of sister chromatids, and mitotic arrest. Evidence suggests that this protein also protects a small subset of cohesin found along the length of the chromosome arms during mitotic prophase. An isoform lacking exon 6 has been shown to play a role in the cohesion of centrioles (PMID: 16582621 and PMID:18331714). Mutations in this gene have been associated with Chronic Atrial and Intestinal Dysrhythmia (CAID) syndrome, characterized by the co-occurrence of Sick Sinus Syndrome (SSS) and Chronic Intestinal Pseudo-obstruction (CIPO) within the first four decades of life (PMID:25282101). Fibroblast cells from CAID patients exhibited both increased cell proliferation and higher rates of senescence. Pseudogenes of this gene have been found on chromosomes 1 and 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015]

Uniprot Description

Plays a central role in chromosome cohesion during mitosis by preventing premature dissociation of cohesin complex from centromeres after prophase, when most of cohesin complex dissociates from chromosomes arms. May act by preventing phosphorylation of the STAG2 subunit of cohesin complex at the centromere, ensuring cohesin persistence at centromere until cohesin cleavage by ESPL1/separase at anaphase. Essential for proper chromosome segregation during mitosis and this function requires interaction with PPP2R1A. Its phosphorylated form is necessary for chromosome congression and for the proper attachment of spindle microtubule to the kinetochore. Necessary for kinetochore localization of PLK1 and CENPF. May play a role in the tension sensing mechanism of the spindle-assembly checkpoint by regulating PLK1 kinetochore affinity. Isoform 3 plays a role in maintaining centriole cohesion involved in controlling spindle pole integrity. Involved in centromeric enrichment of AUKRB in prometaphase.