Mouse anti-Human Cytokeratin 18 Antibody | anti-CK-18 antibody

Anti-Cytokeratin 18 Antibody

Cat. Num. AAA8510538

• Gene Names: KRT18; K18; CK-18; CYK18
• Reactivity: Human
• Applications: Western Blot, Immunofluorescence, ELISA, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
• Synonyms: Cytokeratin 18; Anti-Cytokeratin 18 Antibody; anti-CK-18 antibody

• Host: Mouse
• Reactivity: Human
• Isotype: IgG1
• Clone Number: 4ADA-7
• Form/Format: Phosphate-buffered solution, pH 7.4, containing 0.09% sodium azide
• Sequence Length: 430

• Applicable Applications for anti-CK-18 antibody: Western Blot (WB), Immunofluorescence (IF), ELISA (EIA), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
• Preparation and Storage: Store at 4 degree C. DO NOT FREEZE. LIGHT SENSITIVE MATERIAL.

Western Blot (WB)

(MCF-7 cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-cytokeratin 18 antibody (clone 4ADA-7). Proteinwere visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.)

Related Product Information for anti-CK-18 antibody

Cytokeratin 18, also known as keratin 18, is a type I intermediate filament protein with a molecular weight of approximately 48 kD. Cytokeratin 18 is a heterotetramer composed of two type I and two type II keratins. Cytokeratin 18 associates with cytokeratin 8 and is widely expressed in simple epithelial tissues in the adult where it is found in the cytoplasm, nucleus, and nucleolus. Cytokeratin 18 is associated with the cytoskeleton and mutations in this protein have been associated with liver disease after environmental insult. Cytokeratin 18 can be modified by glycosylation, phosphorylation (Ser34 and Ser53), acetylation (Ser2) and proteolytic cleavage at amino acids 238 and 397 by caspase family members. The cytokeratin 18 protein interacts with a number of proteins including caspase 3, 14-3-3 gamma, 14-3-3 zeta, 14-3-3 sigma 14-3-3 beta, 14-3-3 eta, keratin 5, keratin 8, TNF receptor II, plakophilin 2, EGF receptor, and usherin, among others. The 4ADA-7 monoclonal antibody recognizes human cytokeratin 18 and is useful for Western blotting. This antibody has also been reported to be useful for immunoprecipitation, immunohistochemistry (paraffin sections), immunocytochemistry, and ELISA.

NCBI and Uniprot Product Information

• NCBI GI #: 34037
• NCBI GeneID: 3875
• UniProt Accession #: P05783; Q53G38; Q5U0N8; Q9BW26
• Molecular Weight: 48,058 Da
• NCBI Official Full Name: cytokeratin 18
• NCBI Official Synonym Full Names: keratin 18
• NCBI Official Symbol: KRT18
• NCBI Official Synonym Symbols: K18; CK-18; CYK18
• NCBI Protein Information: keratin, type I cytoskeletal 18
• UniProt Protein Name: Keratin, type I cytoskeletal 18
• UniProt Gene Name: KRT18
• UniProt Synonym Gene Names: CYK18; CK-18; K18

NCBI Description

KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.