NF-kappaBp100/p52 Antibody | anti-NFKB2 antibody

NF-kappaBp100/p52 (Phospho-Ser869)Antibody

Cat. Num. AAA8510215

• Gene Names: NFKB2; p52; p100; H2TF1; LYT10; CVID10; LYT-10; NF-kB2; p49/p100
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, ELISA, Immunoprecipitation
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
• Synonyms: NF-kappaBp100/p52; NF-kappaBp100/p52 (Phospho-Ser869)Antibody; anti-NFKB2 antibody

• Reactivity: Human, Mouse, Rat
• Specificity: NF-kappaB p100/p52 (phospho-Ser869) antibody detects endogenous levels of NF-kappaB p100/p52 only when phosphorylated at serine 869.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 900

• Applicable Applications for anti-NFKB2 antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA), Immunoprecipitation (IP)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:100; ELISA: 1:10000
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human NF-kappaB around the phosphorylation site of serine 869 (Y-G-SP-Q-S).
• Preparation and Storage: Store at-20 degree C for 1 year

Immunohistochemistry (IHC)

(P-Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NF-kappaB p100/p52 (phospho-Ser869) antibody.)

Western Blot (WB)

(Western blot analysis of extracts from MDA-MB-435 cells using NF-kappaB p100/p52 (phospho-Ser869) antibody.)

References

Qu Z, et al. (2004) J Biol Chem; 279(43): 44563-72. Xiao G, et al. (2001) J Biol Chem 7(2): 401-9.

NCBI and Uniprot Product Information

• NCBI GI #: 117320531
• NCBI GeneID: 4791
• NCBI Accession #: NP_001070962.1
• NCBI GenBank Nucleotide #: NM_001077494.3
• UniProt Accession #: Q00653; Q04860; Q9BU75; Q9H471; Q9H472; A8K9D9; D3DR83
• NCBI Official Full Name: nuclear factor NF-kappa-B p100 subunit isoform a
• NCBI Official Synonym Full Names: nuclear factor kappa B subunit 2
• NCBI Official Symbol: NFKB2
• NCBI Official Synonym Symbols: p52; p100; H2TF1; LYT10; CVID10; LYT-10; NF-kB2; p49/p100
• NCBI Protein Information: nuclear factor NF-kappa-B p100 subunit
• UniProt Protein Name: Nuclear factor NF-kappa-B p100 subunit
• Protein Family: Nuclear factor
• UniProt Gene Name: NFKB2
• UniProt Synonym Gene Names: LYT10; Lyt10

NCBI Description

This gene encodes a subunit of the transcription factor complex nuclear factor-kappa-B (NFkB). The NFkB complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function. The protein encoded by this gene can function as both a transcriptional activator or repressor depending on its dimerization partner. The p100 full-length protein is co-translationally processed into a p52 active form. Chromosomal rearrangements and translocations of this locus have been observed in B cell lymphomas, some of which may result in the formation of fusion proteins. There is a pseudogene for this gene on chromosome 18. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]

Uniprot Description

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. In a non-canonical activation pathway, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. The NF-kappa-B heterodimeric RelB-p52 complex is a transcriptional activator. The NF-kappa-B p52-p52 homodimer is a transcriptional repressor. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. p52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. p52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65. In concert with RELB, regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer.