anti-Human, Mouse RPC2 Antibody | anti-RPC2 antibody

RPC2 Antibody

Cat. Num. AAA8509857

• Gene Names: POLR3B; C128; HLD8; RPC2; INMAP
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: RPC2; RPC2 Antibody; DNA-directed RNA polymerase III subunit 127.6 kDa polypeptide; EC 2.7.7.6; polymerase (RNA) III (DNA directed) polypeptide B; RNA polymerase III subunit 2; RNA polymerase III subunit RPC2; anti-RPC2 antibody

• Reactivity: Human, Mouse
• Specificity: RPC2 antibody detects endogenous levels of total RPC2 protein.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 1075

• Applicable Applications for anti-RPC2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; ELISA: 1:20000
• Immunogen: The antiserum was produced against synthesized peptide derived from internal of human RPC2.
• Preparation and Storage: Store at-20 degree C for 1 year.

Western Blot (WB)

(Western blot analysis of extracts from LOVO cells, using RPC2 antibody.)

References

Hu P., Mol. Cell. Biol. 22:8044-8055(2002). The MGC Project Team; Genome Res. 14:2121-2127(2004). Ota T., Nat. Genet. 36:40-45(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 238908505
• NCBI GeneID: 55703
• NCBI Accession #: NP_001154180.1
• NCBI GenBank Nucleotide #: NM_001160708.1
• UniProt Accession #: Q9NW08; Q9NW59; A8K6H0; B3KV73; F5H1E6
• Molecular Weight: 121,120 Da
• NCBI Official Full Name: DNA-directed RNA polymerase III subunit RPC2 isoform 2
• NCBI Official Synonym Full Names: RNA polymerase III subunit B
• NCBI Official Symbol: POLR3B
• NCBI Official Synonym Symbols: C128; HLD8; RPC2; INMAP
• NCBI Protein Information: DNA-directed RNA polymerase III subunit RPC2
• UniProt Protein Name: DNA-directed RNA polymerase III subunit RPC2
• Protein Family: DNA-directed RNA polymerase
• UniProt Gene Name: POLR3B
• UniProt Synonym Gene Names: RNA polymerase III subunit C2

NCBI Description

This gene encodes the second largest subunit of RNA polymerase III, the polymerase responsible for synthesizing transfer and small ribosomal RNAs in eukaryotes. The largest subunit and the encoded protein form the catalytic center of RNA polymerase III. Mutations in this gene are a cause of hypomyelinating leukodystrophy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol III is composed of mobile elements and RPC2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft (). Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.