anti-Human NXF1 Antibody | anti-NXF1 antibody

NXF1 Antibody

Cat. Num. AAA8509796

• Gene Names: NXF1; TAP; MEX67
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: NXF1; NXF1 Antibody; MEX67; TAP; Tip-associated protein; mRNA export factor TAP; nuclear RNA export factor 1; anti-NXF1 antibody

• Reactivity: Human
• Specificity: NXF1 antibody detects endogenous levels of total NXF1 protein.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 356

• Applicable Applications for anti-NXF1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; ELISA: 1:40000
• Immunogen: The antiserum was produced against synthesized peptide derived from N-terminal of human NXF1.
• Preparation and Storage: Store at-20 degree C for 1 year.

Western Blot (WB)

(Western blot analysis of extracts from HepG2 cells, Jurkat cells and HeLa cells, using NXF1 antibody.)

References

Braun I.C., EMBO J. 18:1953-1965(1999). Kang Y., Genes Dev. 13:1126-1139(1999). Bear J., Mol. Cell. Biol. 19:6306-6317(1999).

NCBI and Uniprot Product Information

• NCBI GI #: 125625324
• NCBI GeneID: 10482
• NCBI Accession #: NP_001074960.1
• NCBI GenBank Nucleotide #: NM_001081491.1
• UniProt Accession #: Q9UBU9; Q99799; Q9UQL2; B4E269
• Molecular Weight: 40,476 Da
• NCBI Official Full Name: nuclear RNA export factor 1 isoform 2
• NCBI Official Synonym Full Names: nuclear RNA export factor 1
• NCBI Official Symbol: NXF1
• NCBI Official Synonym Symbols: TAP; MEX67
• NCBI Protein Information: nuclear RNA export factor 1
• UniProt Protein Name: Nuclear RNA export factor 1
• Protein Family: Nuclear RNA export factor
• UniProt Gene Name: NXF1
• UniProt Synonym Gene Names: TAP

NCBI Description

This gene is one member of a family of nuclear RNA export factor genes. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. Alternative splicing seems to be a common mechanism in this gene family. The encoded protein of this gene shuttles between the nucleus and the cytoplasm and binds in vivo to poly(A)+ RNA. It is the vertebrate homologue of the yeast protein Mex67p. The encoded protein overcomes the mRNA export block caused by the presence of saturating amounts of CTE (constitutive transport element) RNA of type D retroviruses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]

Uniprot Description

Involved in the nuclear export of mRNA species bearing retroviral constitutive transport elements (CTE) and in the export of mRNA from the nucleus to the cytoplasm (TAP/NFX1 pathway) (PubMed:10924507). The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with ALYREF/THOC4 and THOC5 components of the TREX complex. ALYREF/THOC4-bound mRNA is thought to be transferred to the NXF1-NXT1 heterodimer for export.