anti-Human, Mouse GTPase Activating Protein Antibody | anti-RACGAP1 antibody

GTPase Activating Protein (Phospho-Ser387) Antibody

Cat. Num. AAA8509719

• Gene Names: RACGAP1; CYK4; ID-GAP; HsCYK-4; MgcRacGAP
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
• Purity: Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Synonyms: GTPase Activating Protein; GTPase Activating Protein (Phospho-Ser387) Antibody; GTPase activating protein; GTPase activating protein ID-GAP; MGCRACGAP variant alpha; MGCRACGAP variant beta; RAC GTPase-activating protein 1; RAC GTPase-activating protein; RACGAP1; anti-RACGAP1 antibody

• Reactivity: Human, Mouse
• Specificity: GTPase Activating Protein (Phospho-Ser387) antibody detects endogenous levels of GTPase Activating Protein only when phosphorylated at serine 387.
• Purity/Purification: Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 632

• Applicable Applications for anti-RACGAP1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:20000
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human GTPase Activating Protein around the phosphorylation site of serine 387 (R-I-SP-G-C).
• All Sites: Human: Ser388; Mouse: Ser388
• Preparation and Storage: Store at-20 degree C for 1 year.

Testing Data

(P-peptide-+)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human placenta tissue using GTPase Activating Protein (Phospho-Ser387) antibody. Western blot analysis of extracts from COS7 cells, using GTPase Activating Protein (Phospho-Ser387) antibody.)

Immunofluorescence (IF)

(P-peptide-+ Immunofluorescence analysis of HeLa cells, using GTPase Activating Protein (Phospho-Ser387) antibody.)

References

Kawashima T., Blood 96:2116-2124(2000). Wiemann S., Genome Res. 11:422-435(2001). Ota T., Nat. Genet. 36:40-45(2004). Nagase T., DNA Res. 7:143-150(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 186910300
• NCBI GeneID: 29127
• NCBI Accession #: NP_001119575.1
• NCBI GenBank Nucleotide #: NM_001126103.2
• UniProt Accession #: Q9H0H5; Q6PJ26; Q9NWN2; Q9P250; Q9P2W2
• Molecular Weight: 71,027 Da
• NCBI Official Full Name: rac GTPase-activating protein 1 isoform a
• NCBI Official Synonym Full Names: Rac GTPase activating protein 1
• NCBI Official Symbol: RACGAP1
• NCBI Official Synonym Symbols: CYK4; ID-GAP; HsCYK-4; MgcRacGAP
• NCBI Protein Information: rac GTPase-activating protein 1
• UniProt Protein Name: Rac GTPase-activating protein 1
• Protein Family: GTPase activating protein
• UniProt Gene Name: RACGAP1
• UniProt Synonym Gene Names: MgcRacGAP; CYK4; HsCYK-4

NCBI Description

This gene encodes a GTPase-activating protein (GAP) that is a compoment of the centralspindlin complex. This protein binds activated forms of Rho GTPases and stimulates GTP hydrolysis, which results in negative regulation of Rho-mediated signals. This protein plays a regulatory role in cytokinesis, cell growth, and differentiation. Alternatively spliced transcript variants have been found for this gene. There is a pseudogene for this gene on chromosome 12. [provided by RefSeq, Feb 2016]

Uniprot Description

Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Required for proper attachment of the midbody to the cell membrane during cytokinesis. Plays key roles in controlling cell growth and differentiation of hematopoietic cells through mechanisms other than regulating Rac GTPase activity. Also involved in the regulation of growth-related processes in adipocytes and myoblasts. May be involved in regulating spermatogenesis and in the RACGAP1 pathway in neuronal proliferation. Shows strong GAP (GTPase activation) activity towards CDC42 and RAC1 and less towards RHOA. Essential for the early stages of embryogenesis. May play a role in regulating cortical activity through RHOA during cytokinesis. May participate in the regulation of sulfate transport in male germ cells.