PYK2 Antibody | anti-PYK2 antibody

PYK2 (Phospho-Tyr580) Antibody

Cat. Num. AAA8509360

• Gene Names: PTK2B; PKB; PTK; CAKB; FAK2; PYK2; CADTK; FADK2; RAFTK
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Synonyms: PYK2; PYK2 (Phospho-Tyr580) Antibody; PTK2B protein tyrosine kinase 2 beta isoform a; PTK2B protein tyrosine kinase 2 beta isoform b; calcium-dependent tyrosine kinase; cell adhesion kinase beta; focal adhesion kinase 2; proline-rich tyrosine kinase 2 protein kinase B; related adhesion focal tyrosine kinase; anti-PYK2 antibody

• Reactivity: Human, Mouse, Rat
• Specificity: PYK2 (phospho-Tyr580) antibody detects endogenous levels of PYK2 only when phosphorylated at tyrosine 580.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 1009

• Applicable Applications for anti-PYK2 antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: WB: 1: 500-1:1000; IHC: 1:50-1:100; ELISA: 1:40000
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human PYK2 around the phosphorylation site of tyrosine 580 (D-Y-YP-K-A).
• All Sites: Human: Tyr580; Mouse: Tyr580; Rat: Tyr580
• Preparation and Storage: Store at-20 degree C for 1 year

Immunohistochemistry (IHC)

(P-peptide-+ Immunohistochemical analysis of paraffin-embedded human brain tissue using PYK2 (phospho-Tyr580) antibody.)

Western Blot (WB)

(Western blot analysis of extracts from K562 cells, using PYK2 (phospho-Tyr580) antibody.)

References

Yoshiko Banno, J. Biol. Chem., Apr 2005; 280: 16319-16324. Robin R. Hodges, Invest. Ophthalmol. Vis. Sci., Aug 2006; 47: 3352-3359. Sarah J. McLeod, J. Biol. Chem., Mar 2004; 279: 12009-12019. Chong-Shan Shi, J. Biol. Chem., Aug 2000; 275: 24470.

NCBI and Uniprot Product Information

• NCBI GI #: 27886584
• NCBI GeneID: 2185
• NCBI Accession #: NP_004094.3
• NCBI GenBank Nucleotide #: NM_004103.4
• UniProt Accession #: Q14289; Q13475; Q14290; Q16709; Q6PID4; D3DST0
• Molecular Weight: 111,183 Da
• NCBI Official Full Name: protein-tyrosine kinase 2-beta isoform a
• NCBI Official Synonym Full Names: protein tyrosine kinase 2 beta
• NCBI Official Symbol: PTK2B
• NCBI Official Synonym Symbols: PKB; PTK; CAKB; FAK2; PYK2; CADTK; FADK2; RAFTK
• NCBI Protein Information: protein-tyrosine kinase 2-beta
• UniProt Protein Name: Protein-tyrosine kinase 2-beta
• Protein Family: Pyruvate kinase
• UniProt Gene Name: PTK2B
• UniProt Synonym Gene Names: FAK2; PYK2; RAFTK; CADTK; CAK-beta; CAKB; FADK 2; RAFTK

NCBI Description

This gene encodes a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Non-receptor protein-tyrosine kinase that regulates reorganization of the actin cytoskeleton, cell polarization, cell migration, adhesion, spreading and bone remodeling. Plays a role in the regulation of the humoral immune response, and is required for normal levels of marginal B-cells in the spleen and normal migration of splenic B-cells. Required for normal macrophage polarization and migration towards sites of inflammation. Regulates cytoskeleton rearrangement and cell spreading in T-cells, and contributes to the regulation of T-cell responses. Promotes osteoclastic bone resorption; this requires both PTK2B/PYK2 and SRC. May inhibit differentiation and activity of osteoprogenitor cells. Functions in signaling downstream of integrin and collagen receptors, immune receptors, G-protein coupled receptors (GPCR), cytokine, chemokine and growth factor receptors, and mediates responses to cellular stress. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and of the AKT1 signaling cascade. Promotes activation of NOS3. Regulates production of the cellular messenger cGMP. Promotes activation of the MAP kinase signaling cascade, including activation of MAPK1/ERK2, MAPK3/ERK1 and MAPK8/JNK1. Promotes activation of Rho family GTPases, such as RHOA and RAC1. Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Acts as a scaffold, binding to both PDPK1 and SRC, thereby allowing SRC to phosphorylate PDPK1 at 'Tyr-9, 'Tyr-373', and 'Tyr-376'. Promotes phosphorylation of NMDA receptors by SRC family members, and thereby contributes to the regulation of NMDA receptor ion channel activity and intracellular Ca2+ levels. May also regulate potassium ion transport by phosphorylation of potassium channel subunits. Phosphorylates SRC; this increases SRC kinase activity. Phosphorylates ASAP1, NPHP1, KCNA2 and SHC1. Promotes phosphorylation of ASAP2, RHOU and PXN; this requires both SRC and PTK2/PYK2.