MEF2A Antibody | anti-MEF2A antibody

MEF2A (Phospho-Ser408) Antibody

Cat. Num. AAA8509315

• Gene Names: MEF2A; mef2; ADCAD1; RSRFC4; RSRFC9
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Synonyms: MEF2A; MEF2A (Phospho-Ser408) Antibody; MEF2; Myocyte-specific enhancer factor 2A; Serum response factor-like protein 1; anti-MEF2A antibody

• Reactivity: Human, Mouse, Rat
• Specificity: MEF2A (phospho-Ser408) antibody detects endogenous levels of MEF2A only when phosphorylated at serine 408.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 437

• Applicable Applications for anti-MEF2A antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
• Application Notes: WB: 1:500~1000; IHC: 1:50~100; IF: 1:100~1:500; ELISA: 1:40000
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human MEF2A around the phosphorylation site of serine 408 (P-I-SP-P-P).
• All Sites: Human: Ser408; Mouse: Ser406; Rat: Ser400
• Preparation and Storage: Store at-20 degree C for 1 year

Immunohistochemistry (IHC)

(P-peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEF2A (phospho-Ser408) antibody.)

Western Blot (WB)

(Western blot analysis of extracts from Hela cells treated with PMA (125ng/ml, 30mins), using MEF2A (phospho-Ser408) antibody.)

Immunofluorescence (IF)

(PMA +- Immunofluorescence analysis of HeLa cells, treated with PMA (125ng/ml, 30mins), using MEF2A (phospho-Ser408) antibody (#11319).)

References

Cyril Rauch, Am J Physiol Cell Physiol, Mar 2005; 288: C593-C605. OI Ornatsky, Nucleic Acids Res., Jul 1999; 27: 2646-2654. David P. Sparling, Am J Physiol Endocrinol Metab, Apr 2007; 292: E1149-E1156. Eric N. Olson Sci, Aging Knowl. Environ., Dec 2003; 2003: 33.

NCBI and Uniprot Product Information

• NCBI GI #: 195972803
• NCBI GeneID: 4205
• NCBI Accession #: NP_001124399.1
• NCBI GenBank Nucleotide #: NM_001130927.2
• UniProt Accession #: Q02078; O43814; Q14223; Q14224; Q59GX4; Q7Z6C9; Q96D14; B4DFQ7; F6XG23
• Molecular Weight: 45,570 Da
• NCBI Official Full Name: myocyte-specific enhancer factor 2A isoform 3
• NCBI Official Synonym Full Names: myocyte enhancer factor 2A
• NCBI Official Symbol: MEF2A
• NCBI Official Synonym Symbols: mef2; ADCAD1; RSRFC4; RSRFC9
• NCBI Protein Information: myocyte-specific enhancer factor 2A
• UniProt Protein Name: Myocyte-specific enhancer factor 2A
• Protein Family: Myocyte-specific enhancer factor
• UniProt Gene Name: MEF2A
• UniProt Synonym Gene Names: MEF2

NCBI Description

The protein encoded by this gene is a DNA-binding transcription factor that activates many muscle-specific, growth factor-induced, and stress-induced genes. The encoded protein can act as a homodimer or as a heterodimer and is involved in several cellular processes, including muscle development, neuronal differentiation, cell growth control, and apoptosis. Defects in this gene could be a cause of autosomal dominant coronary artery disease 1 with myocardial infarction (ADCAD1). Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2010]

Uniprot Description

Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT]4TAR-3', found in numerous muscle-specific genes. Also involved in the activation of numerous growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. In cerebellar granule neurons, phosphorylated and sumoylated MEF2A represses transcription of NUR77 promoting synaptic differentiation. Associates with chromatin to the ZNF16 promoter.