Western Blot (WB)
(Figure 1. Western blot analysis using BRAF mouse mAb against truncated recombinant Braf (1) and A431 cell lysate (2).)
Mouse anti-Human BRAF Antibody | anti-BRAF antibody
BRAF Antibody
IHC: 1:200-1:1000
ELISA: 1:10000
Western Blot (WB)
(Figure 1. Western blot analysis using BRAF mouse mAb against truncated recombinant Braf (1) and A431 cell lysate (2).)
Western Blot (WB)
(Figure 1. Western blot analysis using BRAF mouse mAb against truncated recombinant Braf (1) and A431 cell lysate (2).)
Western Blot (WB)
(Figure 2. Western blot analysis using BRAF mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY Braf cDNA (2).)
Western Blot (WB)
(Figure 2. Western blot analysis using BRAF mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY Braf cDNA (2).)
Immunohistochemistry (IHC)
(Figure 3. Immunohistochemical analysis of paraffin-embedded human bladder carcinoma tissue(left) and lung carcinoma tissue (right) showing cytoplasmic localization using BRAF mouse mAb with DAB staining.)
Immunohistochemistry (IHC)
(Figure 3. Immunohistochemical analysis of paraffin-embedded human bladder carcinoma tissue(left) and lung carcinoma tissue (right) showing cytoplasmic localization using BRAF mouse mAb with DAB staining.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome. [provided by RefSeq, Aug 2017]
Uniprot Description
Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.
Research Articles on BRAF
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Product Notes
The BRAF braf (Catalog #AAA850917) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The BRAF Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BRAF can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:200-1:1000 ELISA: 1:10000. Researchers should empirically determine the suitability of the BRAF braf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BRAF, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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