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Western Blot (WB) (Figure 1: Western blot analysis using CK5 mouse mAb against truncated CK5 recombinant protein)

Mouse anti-Human Cytokeratin 5 Antibody | anti-CK5 antibody

Cytokeratin 5 Antibody

Gene Names
KRT5; K5; CK5; DDD; DDD1; EBS2; KRT5A
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, ELISA
Synonyms
Cytokeratin 5; Cytokeratin 5 Antibody; K5; DDD; EBS2; KRT5A; KRT5; anti-CK5 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Isotype
IgG1
Clone Number
3E2F1
Form/Format
Ascitic fluid containing 0.03% sodium azide.
Sequence Length
590
Applicable Applications for anti-CK5 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:200-1:1000
ELISA: 1:10000
Immunogen
Purified recombinant fragment of CK5 expressed in E. Coli.
Preparation and Storage
Store at 4 degree C, for long term storage, store at-20 degree C

Western Blot (WB)

(Figure 1: Western blot analysis using CK5 mouse mAb against truncated CK5 recombinant protein)

Western Blot (WB) (Figure 1: Western blot analysis using CK5 mouse mAb against truncated CK5 recombinant protein)

Immunohistochemistry (IHC)

(Figure 2: Immunohistochemical analysis of paraffin-embedded human esophagus epithelium (A), salivary gland basal cell (B), lung squamous cell carcinoma (C), endometrium admosquamous carcinoma (D), showing cytoplasmic and membrane localization using CK5 mouse mAb with DAB staining.)

Immunohistochemistry (IHC) (Figure 2: Immunohistochemical analysis of paraffin-embedded human esophagus epithelium (A), salivary gland basal cell (B), lung squamous cell carcinoma (C), endometrium admosquamous carcinoma (D), showing cytoplasmic and membrane localization using CK5 mouse mAb with DAB staining.)

Immunohistochemistry (IHC)

(Figure 3: Immunohistochemical analysis of paraffin-embedded human skin tissues using CK5 mouse mAb.)

Immunohistochemistry (IHC) (Figure 3: Immunohistochemical analysis of paraffin-embedded human skin tissues using CK5 mouse mAb.)
Related Product Information for anti-CK5 antibody
CK5 (keratin 5) is a member of the keratin gene family. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Cytokeratin 5 is expressed in normal basal cells. Mutations of the Cytokeratin5 gene (KRT5) have been shown to result in the autosomal dominant disorderepidermolysis bullosa (EB). Defects in KRT5 are a cause of epidermolysis bullosa simplex.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
62,378 Da
NCBI Official Full Name
keratin, type II cytoskeletal 5
NCBI Official Synonym Full Names
keratin 5
NCBI Official Symbol
KRT5
NCBI Official Synonym Symbols
K5; CK5; DDD; DDD1; EBS2; KRT5A
NCBI Protein Information
keratin, type II cytoskeletal 5
UniProt Protein Name
Keratin, type II cytoskeletal 5
UniProt Gene Name
KRT5
UniProt Synonym Gene Names
CK-5; K5

NCBI Description

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]

Uniprot Description

MiscellaneousThere are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).

Research Articles on CK5

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Product Notes

The CK5 krt5 (Catalog #AAA850589) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Cytokeratin 5 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cytokeratin 5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:200-1:1000 ELISA: 1:10000. Researchers should empirically determine the suitability of the CK5 krt5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytokeratin 5, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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