Rabbit anti-Human p14 ARF Antibody | anti-p14 ARF antibody

p14 ARF (P16) Antibody

Cat. Num. AAA8503130

• Gene Names: CDKN2A; ARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity purification
• Synonyms: p14 ARF; p14 ARF (P16) Antibody; CDKN2A; ARF; CDK4I; CDKN2; CMM2; INK4; INK4a; MLM; MTS1; TP16; p14; p14ARF; p16; p16INK4; p16INK4a; p19; anti-p14 ARF antibody

• Host: Rabbit
• Reactivity: Human
• Isotype: IgG
• Purity/Purification: Affinity purification
• Sequence Length: 156

• Applicable Applications for anti-p14 ARF antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: C term-peptide of human P16
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using P16 antibody.)

Related Product Information for anti-p14 ARF antibody

The division cycle of eukaryotic cells is regulated by a family of protein kinases known as the cyclin-dependent kinases (CDKs). The sequential activation of individual members of this family and their consequent phosphorylation of critical substrates promotes orderly progression through the cell cycle. It has been reported that p16 binds to CDK4 and inhibits the catalytic activity of the CDK4/cyclin D enzymes. p16 seems to act in a regulatory feedback circuit with CDK4, D-type cyclins and retinoblastoma protein (1). The INK4 (inhibitor of cyclin-dependent kinase 4) family consists of four tumor-suppressor proteins: p15(INK4B), p16(INK4A), p18(INK4C), and p19(INK4D). While their sequences and structures are highly homologous, they show appreciable differences in conformational flexibility, stability, and aggregation tendency (2). Cell cycle arrest at the G1 checkpoint allows completion of critical macromolecular events prior to S phase. Regulators of the G1 checkpoint include an inhibitor of cyclin-dependent kinase, p16INK4; two tumor-suppressor proteins, p53 and RB and cyclin D1. p16INK4 is a tumor-suppressor protein and that genetic and epigenetic abnormalities in genes controlling the G1 checkpoint can lead to both escape from senescence and cancer formation (3).

Product Categories/Family for anti-p14 ARF antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

1. Serrano M et al. Nature 366(6456):704-7, 1993 2. Yuan C et al. J MolBiol 294(1):201-11, 1999 3. Okamoto A et al. ProcNatlAcadSci 91(23):11045-9, 1994.

NCBI and Uniprot Product Information

• NCBI GI #: 4502749
• NCBI GeneID: 1029
• NCBI Accession #: NP_000068.1
• NCBI GenBank Nucleotide #: NM_000077.4
• UniProt Accession #: P42771; O95440; Q15191; Q5VVJ5; Q96B52; Q9NP05; A5X2G7; D3DRK1; Q8N726
• Molecular Weight: 11kDa
• NCBI Official Full Name: cyclin-dependent kinase inhibitor 2A isoform p16INK4a
• NCBI Official Synonym Full Names: cyclin dependent kinase inhibitor 2A
• NCBI Official Symbol: CDKN2A
• NCBI Official Synonym Symbols: ARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A
• NCBI Protein Information: cyclin-dependent kinase inhibitor 2A
• UniProt Protein Name: Cyclin-dependent kinase inhibitor 2A
• UniProt Gene Name: CDKN2A
• UniProt Synonym Gene Names: CDK4I; MTS-1; p16-INK4; p16INK4A

NCBI Description

This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, the E3 ubiquitin-protein ligase MDM2, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene. [provided by RefSeq, Sep 2012]

Uniprot Description

Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein.