Rabbit CBLB Antibody | anti-CBLB antibody

CBLB Antibody

Cat. Num. AAA8502778

• Gene Names: CBLB; Cbl-b; RNF56; Nbla00127
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity purification
• Synonyms: CBLB; CBLB Antibody; Cbl-b; RNF56; Nbla00127; anti-CBLB antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Purity/Purification: Affinity purification
• Sequence Length: 982

• Applicable Applications for anti-CBLB antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: Recombinant protein of human CBLB
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.

Testing Data

(Western blot analysis of extracts of various celllines, using CBLB antibody.)

Related Product Information for anti-CBLB antibody

The Casitas B lineage lymphoma (Cbl) proteins (in mammals these are c-Cbl, Cbl-b, and Cbl-c) are a family of single subunit RING finger protein-ubiquitin E3 ligases that contain multiple protein interaction motifs (1). All Cbl proteins have a highly conserved N-terminal tyrosine kinase-binding (TKB) domain that mediates interactions between Cbl proteins and phosphorylated tyrosine residues on Cbl substrates. C-terminal to the RING finger, Cbl proteins have proline-rich domains that mediate interactions with SH3 domain-containing proteins. Phosphorylated tyrosine residues mediate interactions with SH2 domain-containing proteins such as the p85 subunit of PI3K. These protein-protein interaction motifs allow Cbl family proteins to function as adaptor proteins (2). This adaptor function contributes to the E3-dependent activities of Cbl proteins by targeting specific substrates for ubiquitination and degradation. The adaptor function also contributes to non-E3-dependent activities, such as the recruitment of proteins involved in receptor tyrosine kinase internalization, localization of Cbl proteins to specific subcellular compartments, and activation of discrete signaling pathways (1). Cbl-b is an E3 ubiquitin ligase with a domain organization nearly identical to that of c-Cbl. The role of Cbl-b in hematopoietic cell physiology is well documented. Cbl-b expression is important for the downregulation of TCR expression during antigen recognition (2). Cbl-b also acts as a potent negative regulator of the CD28 signaling cascade to Vav and Rac1 through its ability to ubiquitinate the p85 regulatory subunit of PI3K (3, 4). As a critical regulator of clonal anergy in T lymphocytes, Cbl-b mRNA and protein are upregulated in T cells following calcium mobilization and calcineurin activation (5). Cbl-b-deficient T cells are resistant to anergy induction (5). The molecular events governing this phenotype are thought to be linked to defects in the ubiquitination of PLC gamma 1 and PKC theta since the degradation of these signaling molecules, which occurs following restimulation of wild-type anergic T cells, fails to occur in Cbl-b-deficient T cells (5).

Product Categories/Family for anti-CBLB antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

1. Schmidt, M.H. and Dikic, I. (2005) Nat Rev Mol Cell Biol 6, 907-18. 2. Naramura, M. et al. (2002) Nat Immunol 3, 1192-9. 3. Chiang, Y.J. et al. (2000) Nature 403, 216-20. 4. Fang, D. and Liu, Y.C. (2001) Nat Immunol 2, 870-5. 5. Heissmeyer, V. et al. (2004) Nat Immunol 5, 255-65.

NCBI and Uniprot Product Information

• NCBI GI #: 1012443564
• NCBI GeneID: 868
• NCBI Accession #: NP_001308717.1
• NCBI GenBank Nucleotide #: NM_001321788.1
• UniProt Accession #: Q13191; Q13192; Q13193; Q3LIC0; Q63Z43; Q8IVC5; A8K9S7; B7WNM4
• Molecular Weight: 109kDa
• NCBI Official Full Name: E3 ubiquitin-protein ligase CBL-B isoform b
• NCBI Official Synonym Full Names: Cbl proto-oncogene B
• NCBI Official Symbol: CBLB
• NCBI Official Synonym Symbols: Cbl-b; RNF56; Nbla00127
• NCBI Protein Information: E3 ubiquitin-protein ligase CBL-B
• UniProt Protein Name: E3 ubiquitin-protein ligase CBL-B
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: CBLB
• UniProt Synonym Gene Names: RNF56

NCBI Description

This gene encodes an E3 ubiquitin-protein ligase which promotes proteosome-mediated protein degradation by transferring ubiquitin from an E2 ubiquitin-conjugating enzyme to a substrate. The encoded protein is involved in the regulation of immune response by limiting T-cell receptor, B-cell receptor, and high affinity immunoglobulin epsilon receptor activation. Studies in mouse suggest that this gene is involved in antifungal host defense and that its inhibition leads to increased fungal killing. Manipulation of this gene may be beneficial in implementing immunotherapies for a variety of conditions, including cancer, autoimmune diseases, allergies, and infections. [provided by RefSeq, Sep 2017]

Uniprot Description

E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Negatively regulates TCR (T-cell receptor), BCR (B-cell receptor) and FCER1 (high affinity immunoglobulin epsilon receptor) signal transduction pathways. In naive T-cells, inhibits VAV1 activation upon TCR engagement and imposes a requirement for CD28 costimulation for proliferation and IL-2 production. Also acts by promoting PIK3R1/p85 ubiquitination, which impairs its recruitment to the TCR and subsequent activation. In activated T-cells, inhibits PLCG1 activation and calcium mobilization upon restimulation and promotes anergy. In B-cells, acts by ubiquitinating SYK and promoting its proteasomal degradation. Slightly promotes SRC ubiquitination. May be involved in EGFR ubiquitination and internalization. May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3.