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Typical Testing Data/Standard Curve (for reference only)

Human S100B ELISA Kit

S100B (Human) ELISA Kit

Reactivity
Human
Synonyms
S100B; S100B (Human) ELISA Kit; S100-B; NEF; S100Beta; S100 Calcium Binding Protein B; S100B elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This kit recognizes Human S100B in samples. No Significant cross-reactivity or interference between Human S100B and analogues was observed.
Assay Type
Sandwich
Detection Range
31.25-2000 pg/ml
Sensitivity
18.75 pg/ml
Preparation and Storage
Store at 4 degree C
Shelf life: ~12 months

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for S100B elisa kit
Background/Introduction: S100 proteins regulate a variety of cellular processes such as cell growth and motility, cell cycle progression, transcription, and differentiation. Research studies have demonstrated that significant correlation exists between aberrant S100 protein expression and cancer progression. S100 proteins primarily mediate immune responses in various tissue types but are also involved in neuronal development. S100B (Human) ELISA kit is based on Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human S100B. Standards or samples are added to the micro ELISA plate wells that bind to the specific antibody. Then a biotinylated detection antibody specific for Human S100B and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. The wells are washed, a TMB substrate solution is added to the wells and blue color develops in proportion to the amount of S100B bound. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human S100B. The concentration of Human S100B in the samples can be calculated by comparing the OD of the samples to the standard curve.
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Product Notes

The Human S100B (Catalog #AAA849459) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA849459 ELISA Kit recognizes Human S100B. It is sometimes possible for the material contained within the vial of "S100B, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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