Rabbit anti-Mouse, Human ERCC1 Polyclonal Antibody | anti-ERCC1 antibody

Anti-ERCC1 Antibody

Cat. Num. AAA847760

• Gene Names: ERCC1; UV20; COFS4; RAD10
• Reactivity: Mouse, Human
• Applications: Immunofluorescence, Western Blot
• Purity: Affinity purification
• Synonyms: ERCC1; Polyclonal Antibody; Anti-ERCC1 Antibody; COFS4; RAD10; UV20; DNA excision repair protein ERCC-1; anti-ERCC1 antibody

• Host: Rabbit
• Reactivity: Mouse, Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Affinity purified rabbit polyclonal antibody to ERCC1
• Purity/Purification: Affinity purification
• Form/Format: Liquid. In PBS with 0.02% sodium azide, 50% glycerol, pH7.3

• Applicable Applications for anti-ERCC1 antibody: Immunofluorescence (IF), Western Blot (WB)
• Application Notes: IF 1:50-1:200; WB: 1:500-1:2000
• Immunogen: Recombinant fusion protein corresponding to aa1-323 of human ERCC1
• Preparation and Storage: Store at -20 degree C; The antibody solution should be gently mixed before use.; Shelf life: ~12 months

Immunocytochemistry/Immunofluorescence (ICC/IF))

Immunocytochemistry/Immunofluorescence (ICC/IF))

Western Blot (WB)

Related Product Information for anti-ERCC1 antibody

The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand.

Product Categories/Family for anti-ERCC1 antibody

Cancer Research; Tumor Biomarkers; Antibodies; Antibodies & Supporting Tools; Primary Antibodies (A-Z); Epigenetics; Epigenetic Antibodies (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 260593723
• NCBI GeneID: 2067
• NCBI Accession #: NP_973730.1
• NCBI GenBank Nucleotide #: NM_202001
• UniProt Accession #: P07992; Q7Z7F5; Q96S40; B2RC01; B3KRR0
• Molecular Weight: 25,211 Da
• NCBI Official Full Name: Homo sapiens excision repair cross-complementation group 1 (ERCC1), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: excision repair cross-complementation group 1
• NCBI Official Symbol: ERCC1
• NCBI Official Synonym Symbols: UV20; COFS4; RAD10
• NCBI Protein Information: DNA excision repair protein ERCC-1; excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence)
• UniProt Protein Name: DNA excision repair protein ERCC-1
• Protein Family: DNA excision repair protein
• UniProt Gene Name: ERCC1
• UniProt Entry Name: ERCC1_HUMAN

NCBI Description

The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand. [provided by RefSeq, Oct 2009]

Uniprot Description

Function: Structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair.

Subunit structure: Heterodimer composed of ERCC1 and XPF/ERRC4.

Subcellular location: Nucleus.

Involvement in disease: Cerebro-oculo-facio-skeletal syndrome 4 (COFS4) [MIM:610758]: A disorder of prenatal onset characterized by microcephaly, congenital cataracts, facial dysmorphism, neurogenic arthrogryposis, growth failure and severe psychomotor retardation. COFS is considered to be part of the nucleotide-excision repair disorders spectrum that include also xeroderma pigmentosum, trichothiodystrophy and Cockayne syndrome.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.10

Sequence similarities: Belongs to the ERCC1/RAD10/SWI10 family.