Typical Testing Data/Standard Curve (for reference only)
Rabbit Anterior Gradient Homolog 2 ELISA Kit | AGR2 elisa kit
Rabbit Anterior Gradient Homolog 2 ELISA Kit
Gene Names
AGR2; AG2; GOB-4; HAG-2; XAG-2; PDIA17; HEL-S-116
Reactivity
Rabbit
Synonyms
Anterior Gradient Homolog 2; Rabbit Anterior Gradient Homolog 2 ELISA Kit; AGR2 elisa kit
Reactivity
Rabbit
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Sandwich
Detection Range
0.5-10ng/mL
Sensitivity
0.1ng/mL
Intended Uses
This AGR2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit AGR2. This ELISA kit for research use only, not for therapeutic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for AGR2 elisa kit
Principle of the assay: AGR2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AGR2 antibody and an AGR2-HRP conjugate. The assay sample and buffer are incubated together with AGR2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AGR2 concentration since AGR2 from samples and AGR2-HRP conjugate compete for the anti-AGR2 antibody binding site. Since the number of sites is limited, as more sites are occupied by AGR2 from the sample, fewer sites are left to bind AGR2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AGR2 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for AGR2 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
19,979 Da
NCBI Official Full Name
anterior gradient protein 2 homolog
NCBI Official Synonym Full Names
anterior gradient 2
NCBI Official Symbol
AGR2
NCBI Official Synonym Symbols
AG2; GOB-4; HAG-2; XAG-2; PDIA17; HEL-S-116
NCBI Protein Information
anterior gradient protein 2 homolog; AG-2; HPC8; anterior gradient homolog 2; secreted cement gland homolog; epididymis secretory protein Li 116; secreted cement gland protein XAG-2 homolog; protein disulfide isomerase family A, member 17
UniProt Protein Name
Anterior gradient protein 2 homolog
Protein Family
UniProt Gene Name
AGR2
UniProt Synonym Gene Names
AG2; UNQ515/PRO1030; AG-2; hAG-2
UniProt Entry Name
AGR2_HUMAN
Uniprot Description
AGR2: Required for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells. Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth. Interacts with LYPD3 and DAG1 (alphaDAG1). Interacts with MUC2; disulfide-linked. Expressed strongly in trachea, lung, stomach, colon, prostate and small intestine. Expressed weakly in pituitary gland, salivary gland, mammary gland, bladder, appendix, ovary, fetal lung, uterus, pancreas, kidney, fetal kidney, testis, placenta, thyroid gland and in estrogen receptor (ER)-positive breast cancer cell lines. Belongs to the AGR family.
Protein type: Oncoprotein; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 7p21.3
Cellular Component: mitochondrion; endoplasmic reticulum; extracellular region
Molecular Function: protein binding
Research Articles on AGR2
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Product Notes
The Rabbit AGR2 agr2 (Catalog #AAA725366) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA725366 ELISA Kit recognizes Rabbit AGR2. It is sometimes possible for the material contained within the vial of "Anterior Gradient Homolog 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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