KIM3/HAVCR2 Active Protein | HAVCR2 active protein

Human CellExp KIM3 / HAVCR2, human recombinant

Cat. Num. AAA845358

• Gene Names: HAVCR2; TIM3; CD366; KIM-3; TIMD3; Tim-3; TIMD-3; HAVcr-2
• Applications: SDS-Page
• Purity: >=97%
• Synonyms: KIM3/HAVCR2; Human CellExp KIM3 / HAVCR2; human recombinant; HAVCR2; TIM3; TIMD3; FLJ14428; KIM3; HAVCR2 active protein

• Host: HEK293 cells
• Purity/Purification: >=97%
• Form/Format: Lyophilized from 0.22 mum filtered solution in 50 mM Tris, 100 mM glycine, pH 7.0. Normally Mannitol or Trehalose is added as protectants before lyophilization.; Appearance: Lyophilized
• Sequence Length: 301

• Applicable Applications for HAVCR2 active protein: SDS-PAGE
• Solubility/Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 ug/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 month. For extended storage, it is recommended to store at -20 degree C.
• Endotoxin Level: <1 EU/mug by LAL method
• Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized rh-Galectin-9 at 500 ng/ml can bind rhKIM3-Fc with an apparent KD<30 nM.
• Results: Measured by its binding ability in a functional ELISA. Immobilized rh-Galectin-9 at 500 ng/ml can bind rhKIM3-Fc with an apparent KD<30 nM.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for HAVCR2 active protein

Background: Hepatitis A virus cellular receptor 2 also known as HAVCR2, FLJ14428, KIM3, TIM3, TIMD3, is a member of the TIM family of immune regulating molecules with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells and their associated cytokines are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. The 2 types of cells also cross-regulate the functions of the other. HAVCR2 is a Th1-specific cell surface protein that regulates macrophage activation and enhances the severity of experimental autoimmune encephalomyelitis in mice. HAVCR2 regulates macrophage activation. Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. May be also involved in T-cell homing. Dysregulation of the HAVCR2-galectin-9 pathway could underlie chronic autoimmune disease states in human, such as multiple sclerosis.

Product Categories/Family for HAVCR2 active protein

Proteins and Enzymes; Human cell expressed recombinant proteinsProteins and Enzymes; Proteins and Enzymes (A-Z) Proteins and Enzymes; Other Proteins & Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 49574534
• NCBI GeneID: 84868
• NCBI Accession #: NP_116171.3
• NCBI GenBank Nucleotide #: NM_032782.4
• UniProt Accession #: Q8TDQ0; Q8WW60; Q96K94; B2RAY2
• Molecular Weight: This protein is fused with the Fc region of human IgG1 at the C-terminus and has a calculated MW of 42 kDa after removal of signal peptide. In DTT-reduced SDS-PAGE, rhKIM3-Fc protein migrates as 45 kDa poly peptide due to glycosylation.
• NCBI Official Full Name: hepatitis A virus cellular receptor 2
• NCBI Official Synonym Full Names: hepatitis A virus cellular receptor 2
• NCBI Official Symbol: HAVCR2
• NCBI Official Synonym Symbols: TIM3; CD366; KIM-3; TIMD3; Tim-3; TIMD-3; HAVcr-2
• NCBI Protein Information: hepatitis A virus cellular receptor 2
• UniProt Protein Name: Hepatitis A virus cellular receptor 2
• Protein Family: Hepatitis A virus cellular receptor
• UniProt Gene Name: HAVCR2
• UniProt Synonym Gene Names: TIM3; TIMD3; HAVcr-2; TIMD-3; TIM-3
• UniProt Entry Name: HAVR2_HUMAN

NCBI Description

The protein encoded by this gene belongs to the immunoglobulin superfamily, and TIM family of proteins. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas, Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. This protein is a Th1-specific cell surface protein that regulates macrophage activation, and inhibits Th1-mediated auto- and alloimmune responses, and promotes immunological tolerance. [provided by RefSeq, Sep 2011]

Uniprot Description

TIM-3: Regulates macrophage activation. Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. May be also involved in T-cell homing. Receptor for LGALS9. Belongs to the immunoglobulin superfamily. TIM family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q33.3

Cellular Component: integral to membrane

Disease: Rheumatoid Arthritis