UCHL5 active protein

Human Recombinant UCHL5

Cat. Num. AAA845238

• Gene Names: UCHL5; UCH37; CGI-70; INO80R; UCH-L5
• Applications: SDS-Page
• Purity: >=90%
• Synonyms: UCHL5; Human Recombinant UCHL5; Ubiquitin carboxyl-terminal hydrolase isozyme L5; UCH37; UCHL5 active protein

• Host: E Coli
• Purity/Purification: >=90%
• Form/Format: 0.5 mg/ml solution in 20 mM Tris-HCl (pH 8.0) containing 5 mM DTT, 200 mM NaCl, 0.1 mM PMSF, 2 mM EDTA and 30% glycerol.; Appearance: Liquid
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MTGNAGEWCL MESDPGVFTE LIKGFGCRGA QVEEIWSLEP ENFEKLKPVH GLIFLFKWQP GEEPAGSVVQ DSRLDTIFFA KQVINNACAT QAIVSVLLNC THQDVHLGET LSEFKEFSQS FDAAMKGLAL SNSDVIRQVH NSFARQQMFE FDTKTSAKEE DAFHFVSYVP VNGRLYELDG LREGPIDLGA CNQDDWISAV RPVIEKRIQK YSEGEIRFNL MAIVSDRKMI YEQKIAELQR QLAEEEPMDT DQGNSMLSAI QSEVAKNQML IEEEVQKLKR YKIENIRRKH NYLPFIMELL KTLAEHQQLI PLVEKAKEKQ NAKKAQETK
• Sequence Length: 328

• Applicable Applications for UCHL5 active protein: SDS-PAGE
• Biological Activity: Specific activity: >500 pmole/min/ug. Measured by the hydrolysis of Ubiquitin-AMC at pH 8.0, at 37 degree C.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -80 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for UCHL5 active protein

Background: UCHL5 is a member of the peptidase C12 family. It is a protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains, and is the deubiquitinating enzyme related to the 19S regulatory subunit of the 26S proteasome.

Product Categories/Family for UCHL5 active protein

Proteins and Enzymes; Proteins and Enzymes (A-Z)  

NCBI and Uniprot Product Information

• NCBI GI #: 312922359
• NCBI GeneID: 51377
• NCBI Accession #: NP_001186190.1
• NCBI GenBank Nucleotide #: NM_001199261.1
• UniProt Accession #: Q9Y5K5; Q5LJA6; Q5LJA7; Q8TBS4; Q96BJ9; Q9H1W5; Q9P0I3; Q9UQN2
• Molecular Weight: 39.7 kDa (349 aa, 1-329 aa + NT His Tag)
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase isozyme L5 isoform 2
• NCBI Official Synonym Full Names: ubiquitin carboxyl-terminal hydrolase L5
• NCBI Official Symbol: UCHL5
• NCBI Official Synonym Symbols: UCH37; CGI-70; INO80R; UCH-L5
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase isozyme L5
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase isozyme L5
• Protein Family: Ubiquitin carboxyl-terminal hydrolase
• UniProt Gene Name: UCHL5
• UniProt Synonym Gene Names: UCH37; UCH-L5
• UniProt Entry Name: UCHL5_HUMAN

Uniprot Description

UCHL5: Protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. Putative regulatory component of the INO80 complex; however is inactive in the INO80 complex and is activated by a transient interaction of the INO80 complex with the proteasome via ADRM1. Belongs to the peptidase C12 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.19.12; Ubiquitin-specific protease; Protease; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: proteasome complex; cytosol; nucleus

Molecular Function: omega peptidase activity; protein binding; endopeptidase inhibitor activity; ubiquitin-specific protease activity

Biological Process: ubiquitin-dependent protein catabolic process; protein deubiquitination; regulation of transcription, DNA-dependent; transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; midbrain development; DNA repair; forebrain morphogenesis; negative regulation of transforming growth factor beta receptor signaling pathway; lateral ventricle development; DNA recombination