EpCAM/CD326 Active Protein | EPCAM active protein

Human CellExp EpCAM / CD326, human recombinant

Cat. Num. AAA845150

• Gene Names: EPCAM; ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1
• Applications: SDS-Page
• Purity: >=95%
• Synonyms: EpCAM/CD326; Human CellExp EpCAM / CD326; human recombinant; EPCAM; DIAR5; DIAR-5; EGP-2; EGP2; EGP314; EGP-314; EGP40; EGP-40; ESA; GA733-2; HNPCC8; HNPCC-8; KS1/4; KSA; M4S1; MIC18; MIC-18; MK-1; MK1; TACSTD1; TACSTD-1; TROP1; TROP-1; CD326; CD-326; Epithelial cell adhesion molecule; tumor-associated calcium signal transducer 1; cluster of differentiation 326.; EPCAM active protein

• Host: HEK293 cells
• Purity/Purification: >=95%
• Form/Format: Lyophilized from 0.22 mum filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.; Appearance: Lyophilized
• Sequence Length: 314

• Applicable Applications for EPCAM active protein: SDS-PAGE
• Solubility/Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 ug/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 month. For extended storage, it is recommended to store at -20 degree C.
• Endotoxin Level: <1 EU/mug by LAL method
• Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized recombinant human Cathepsin V at 10 mug/mL (100 mul/well) can bind biotinylated EpCAM. The EC50 of biotinylated EpCAM is 50-70 ng/mL.
• Results: Measured by its binding ability in a functional ELISA. Immobilized recombinant human Cathepsin V at 10 mug/mL (100 mul/well) can bind biotinylated EpCAM. The EC50 of biotinylated EpCAM is 50-70 ng/mL.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for EPCAM active protein

Background: EpCAM is also known as CO171A,; EGP; EGP40; GA7332; KSA; M4S; MIC18; MK1; TROP1; hEGP2, and is a pan-epithelial differentiation antigen that is expressed on almost all carcinomas as 17-1A(mAb) antigen. Its constitutional function is being elucidated. It is intricately linked with the Cadherin-Catenin pathway and hence the fundamental WNT pathway responsible for intracellular signaling and polarity. The epithelial cell adhesion molecule (Ep-CAM) is known to express in most epithelial malignancies and was reported as a tumor marker or a candidate of molecular targeting therapy. Ep-CAM cross signaling with N-cadherin involves Pi3K, resulting in the abrogation of the cadherin adhesion complexes in epithelial cells was reported. And epithelial cell adhesion molecule (Ep-CAM) recently received increased attention as a prognostic factor in breast cancer.

Product Categories/Family for EPCAM active protein

Cytokines; Growth Factors & Hormones; Human cell expressed recombinant proteins Proteins and Enzymes; Human cell expressed recombinant proteinsProteins and Enzymes; Cytokines; Growth Factors & Hormones; CDsProteins and Enzymes; Cytokines; Growth Factors &

NCBI and Uniprot Product Information

• NCBI GI #: 218505670
• NCBI GeneID: 4072
• NCBI Accession #: NP_002345.2
• NCBI GenBank Nucleotide #: NM_002354.2
• UniProt Accession #: P16422; P18180; Q6FG26; Q6FG49; Q96C47; Q9UCD0
• Molecular Weight: This protein contains a poly histidine tag at C-terminus and has a calculated MW of 29 kDa after removal of signal peptide. The predicted N-terminus is Gln24. In DTT-reduced SDS-PAGE, rh EpCAM protein migrates as 33-36 kDa poly peptide due to glycosylatio
• NCBI Official Full Name: epithelial cell adhesion molecule
• NCBI Official Synonym Full Names: epithelial cell adhesion molecule
• NCBI Official Symbol: EPCAM
• NCBI Official Synonym Symbols: ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1
• NCBI Protein Information: epithelial cell adhesion molecule
• UniProt Protein Name: Epithelial cell adhesion molecule
• Protein Family: Epithelial cell adhesion molecule
• UniProt Gene Name: EPCAM
• UniProt Synonym Gene Names: GA733-2; M1S2; M4S1; MIC18; TACSTD1; TROP1; Ep-CAM; EGP; EGP314; hEGP314
• UniProt Entry Name: EPCAM_HUMAN

NCBI Description

This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]

Uniprot Description

EPCAM: a single-pass type I membrane protein of the EPCAM family. May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinomas including pancreatic carcinoma. May be a marker of cancer stem cells.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2p21

Cellular Component: cell surface; tight junction; basolateral plasma membrane; apical plasma membrane; plasma membrane; integral to membrane; lateral plasma membrane

Molecular Function: protein binding; protein complex binding

Biological Process: ureteric bud development; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; stem cell differentiation

Disease: Diarrhea 5, With Tufting Enteropathy, Congenital; Colorectal Cancer, Hereditary Nonpolyposis, Type 8