MDH1 active protein

Human Recombinant MDH1

Cat. Num. AAA845028

• Gene Names: MDH1; MDHA; MOR2; MDH-s; HEL-S-32; MGC:1375
• Applications: SDS-Page
• Purity: >=95%
• Synonyms: MDH1; Human Recombinant MDH1; Malate dehydrogenase cytoplasmic; MDH-s; MDHA; MOR2; MDH1 active protein

• Host: E Coli
• Purity/Purification: >=95%
• Form/Format: 1 mg/ml solution in 20 mM Tris-HCl (pH 8.0) containing 10% glycerol.; Appearance: Liquid
• Concentration: 1 mg/ml (varies by lot)
• Sequence: MSEPIRVLVT GAAGQIAYSL LYSIGNGSVF GKDQPIILVL LDITPMMGVL DGVLMELQDC ALPLLKDVIA TDKEDVAFKD LDVAILVGSM PRREGMERKD LLKANVKIFK SQGAALDKYA KKSVKVIVVG NPANTNCLTA SKSAPSIPKE NFSCLTRLDH NRAKAQIALK LGVTANDVKN VIIWGNHSST QYPDVNHAKV KLQGKEVGVY EALKDDSWLK GEFVTTVQQR GAAVIKARKL SSAMSAAKAI CDHVRDIWFG TPEGEFVSMG VISDGNSYGV PDDLLYSFPV VIKNKTWKFV EGLPINDFSR EKMDLTAKEL TEEKESAFEF LSSALEHHHH HH
• Sequence Length: 352

• Applicable Applications for MDH1 active protein: SDS-PAGE
• Biological Activity: Specific activity is > 8 units/mg, and is defined as the amount of enzyme that cleaves 1 umole of oxaloacetate and beta-NADH to L-malate and beta-NAD per minute at pH 7.5 at 25 degree C.
• Unit Definition: Specific activity is defined as the amount of enzyme that cleaves 1 umole of oxaloacetate and beta-NADH to L-malate and beta-NAD per minute at pH 7.5 at 25 degree C.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -80 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for MDH1 active protein

Background: Malate Dehydrogenase, Cytoplasmic (MDH1) is an enzyme which belongs to the MDH Type 2 sub-family of LDH/MDH superfamily. MDH1 is involved in the Citric Acid Cycle that catalyzes the conversion of Malate into Oxaloacetate (using NAD+) and vice versa. MDH1 should not be confused with Malic Enzyme, which catalyzes the conversion of Malate to Pyruvate, producing NADPH. MDH1 also participates in Gluconeogenesis, the synthesis of Glucose from smaller molecules. Pyruvate in the mitochondria is acted upon by Pyruvate Carboxylase to form Oxaloacetate, a Citric Acid Cycle intermediate. In order to transport the Oxaloacetate out of the Mitochondria, Malate Dehydrogenase reduces it to Malate, and it then traverses the inner mitochondrial membrane. Once in the cytosol, the Malate is oxidized back to Oxaloacetate by MDH1. Finally, Phosphoenol-Pyruvate Carboxy Kinase (PEPCK) converts Oxaloacetate to Phosphoenol Pyruvate.

Product Categories/Family for MDH1 active protein

Proteins and Enzymes; Proteins and Enzymes (A-Z)  

NCBI and Uniprot Product Information

• NCBI GI #: 312283701
• NCBI GeneID: 4190
• NCBI Accession #: NP_001186040.1
• NCBI GenBank Nucleotide #: NM_001199111.1
• UniProt Accession #: P40925; Q6I9V0; B2R5V5; B4DUN2; B7Z3I7; F5H098
• Molecular Weight: 37.4 kDa (342 aa, 1-334 aa + CT His Tag)
• NCBI Official Full Name: malate dehydrogenase, cytoplasmic isoform 1
• NCBI Official Synonym Full Names: malate dehydrogenase 1, NAD (soluble)
• NCBI Official Symbol: MDH1
• NCBI Official Synonym Symbols: MDHA; MOR2; MDH-s; HEL-S-32; MGC:1375
• NCBI Protein Information: malate dehydrogenase, cytoplasmic
• UniProt Protein Name: Malate dehydrogenase, cytoplasmic
• Protein Family: Malate dehydrogenase
• UniProt Gene Name: MDH1
• UniProt Synonym Gene Names: MDHA
• UniProt Entry Name: MDHC_HUMAN

NCBI Description

Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq, Nov 2010]

Uniprot Description

MDH1: Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq, Nov 2010]

Protein type: Carbohydrate Metabolism - glyoxylate and dicarboxylate; Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - citrate (TCA) cycle; EC 1.1.1.96; EC 1.1.1.37; Oxidoreductase

Chromosomal Location of Human Ortholog: 2p13.3

Cellular Component: extracellular space; centrosome; mitochondrion; cytoplasm; cytosol

Molecular Function: malic enzyme activity; L-malate dehydrogenase activity; diiodophenylpyruvate reductase activity; NAD binding

Biological Process: malate metabolic process; oxaloacetate metabolic process; NADH metabolic process; tricarboxylic acid cycle; carbohydrate metabolic process; glucose metabolic process; pathogenesis; cellular carbohydrate metabolic process; gluconeogenesis