PNP active protein

PNP, human recombinant

Cat. Num. AAA844969

• Gene Names: PNP; NP; PUNP; PRO1837
• Applications: SDS-Page
• Purity: >=90%
• Synonyms: PNP; human recombinant; Purine nucleoside phosphorylase; NP; Inosine phosphorylase; PRO1837; PUNP; PNP active protein

• Host: E Coli
• Purity/Purification: >=90%
• Form/Format: 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2 mM DTT and 0.1 M NaCl; Appearance: Liquid
• Concentration: 1 mg/ml (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MENGYTYEDY KNTAEWLLSH TKHRPQVAII CGSGLGGLTD KLTQAQIFDY GEIPNFPRST VPGHAGRLVF GFLNGRACVM MQGRFHMYEG YPLWKVTFPV RVFHLLGVDT LVVTNAAGGL NPKFEVGDIM LIRDHINLPG FSGQNPLRGP NDERFGDRFP AMSDAYDRTM RQRALSTWKQ MGEQRELQEG TYVMVAGPSF ETVAECRVLQ KLGADAVGMS TVPEVIVARH CGLRVFGFSL ITNKVIMDYE SLEKANHEEV LAAGKQAAQK LEQFVSILMA SIPLPDKAS
• Sequence Length: 289

• Applicable Applications for PNP active protein: SDS-PAGE
• Biological Activity: Specific activity is > 40,000 pmol/min/ug. One unit will form 1.0 pmole of 7-Methyl-6-thioguanosine to 7-Methyl-6-thioguanine per minute at PH 7.5 at 25 degree C
• Results: Specific activity is > 40,000 pmol/min/ug
• Unit Definition: One unit will form 1.0 pmole of 7-Methyl-6-thioguanosine to 7-Methyl-6-thioguanine per minute at PH 7.5 at 25 degree C
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for PNP active protein

Background: PNP belongs to the PNP/MTAP phosphorylase family of proteins. This protein catalyzes the reversible phosphorolysis of ribonucleosides and 2'-deoxyribonucleosides with specificity for guanine, hypoxanthine and their analogs. PNP deficiency is a rare autosomal recessive genetic disease associated with a severe defect in T-lymphocyte function and neurologic disorder in children, comprising four percent of combined immunodeficiency cases. Recombinant human PNP protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques

Product Categories/Family for PNP active protein

Proteins and Enzymes; Proteins and Enzymes (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 157168362
• NCBI GeneID: 4860
• NCBI Accession #: NP_000261.2
• NCBI GenBank Nucleotide #: NM_000270.3
• UniProt Accession #: P00491; Q15160; Q5PZ03; B2R8S5; D3DS00
• Molecular Weight: 34.2 kDa (309 aa, 1-289 aa + His Tag)
• NCBI Official Full Name: purine nucleoside phosphorylase
• NCBI Official Synonym Full Names: purine nucleoside phosphorylase
• NCBI Official Symbol: PNP
• NCBI Official Synonym Symbols: NP; PUNP; PRO1837
• NCBI Protein Information: purine nucleoside phosphorylase
• UniProt Protein Name: Purine nucleoside phosphorylase
• Protein Family: Polyribonucleotide nucleotidyltransferase
• UniProt Gene Name: PNP
• UniProt Synonym Gene Names: NP; PNP
• UniProt Entry Name: PNPH_HUMAN

NCBI Description

This gene encodes an enzyme which reversibly catalyzes the phosphorolysis of purine nucleosides. The enzyme is trimeric, containing three identical subunits. Mutations which result in nucleoside phosphorylase deficiency result in defective T-cell (cell-mediated) immunity but can also affect B-cell immunity and antibody responses. Neurologic disorders may also be apparent in patients with immune defects. A known polymorphism at aa position 51 that does not affect enzyme activity has been described. A pseudogene has been identified on chromosome 2. [provided by RefSeq, Jul 2008]

Uniprot Description

NP: a metabolic enzyme with purine-nucleoside phosphorylase activity. Defects are the cause of nucleoside phosphorylase deficiency (NP deficiency), with severe T-cell immunodeficiency with neurologic disorder in children.

Protein type: Nucleotide Metabolism - pyrimidine; Nucleotide Metabolism - purine; Transferase; EC 2.4.2.1; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide

Chromosomal Location of Human Ortholog: 14q13.1

Cellular Component: cytoskeleton; cytoplasm; intracellular; cytosol

Molecular Function: purine binding; nucleoside binding; drug binding; phosphate binding; purine-nucleoside phosphorylase activity

Biological Process: response to drug; nicotinamide riboside catabolic process; nucleobase, nucleoside and nucleotide metabolic process; inosine catabolic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; purine nucleotide catabolic process; positive regulation of T cell proliferation; immune response; purine base metabolic process; purine salvage; positive regulation of alpha-beta T cell differentiation

Disease: Purine Nucleoside Phosphorylase Deficiency