Nucleoside phosphorylase/PNP Recombinant Protein | PNP recombinant protein

Nucleoside phosphorylase/PNP Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121085

• Gene Names: PNP; NP; PUNP; PRO1837
• Purity: >97% as determined by SDS-PAGE
• Synonyms: Nucleoside phosphorylase/PNP; Nucleoside phosphorylase/PNP Protein; Human; Recombinant (His Tag); Human Purine nucleoside phosphorylase/PNP Protein (His Tag); NP Protein; PRO1837 Protein; PUNP Protein; purine nucleoside phosphorylase; PNP recombinant protein

• Host: E Coli
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, 25% glycerol, pH7.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ser289

• Species: Human
• Predicted N Terminal: Met
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human PNP (P00491)(Met 1-Ser 289) was fused with a polyhistide tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for PNP recombinant protein

Background: Purine nucleoside phosphorylase (PNP) is a purine-metabolizing enzyme that catalyzes the reversible phosphorolysis of 6-oxypurine (deoxy)nucleosides to their respective bases and (deoxy)ribose-1-phosphate. It is a key enzyme in the purine salvage pathway of mammalian cells. Purine nucleoside phosphorylase is a transferase that catalyzes the addition of phosphate and removal of a purine base from guanosine and similar nucleosides.PNP defects result in metabolic abnormalities and fatal T cell immunodeficiency. Purine nucleoside phosphorylase (PNP) is a target for leukemia, gout, and autoimmune disorders.

References

de Azevedo,W.F. et al., 2003, Biochem Biophys Res Commun. 312 (3): 767-72. Canduri,F. et al., 2005, Acta Crystallogr D Biol Crystallogr. 61 (Pt 7): 856-62. Perera,G.K. et al., 2005,Clin Exp Dermatol. 30 (1):27-9.

NCBI and Uniprot Product Information

• NCBI GI #: 157168362
• NCBI GeneID: 4860
• NCBI Accession #: NP_000261.2
• NCBI GenBank Nucleotide #: NM_000270.3
• UniProt Accession #: P00491; Q15160; Q5PZ03; B2R8S5; D3DS00
• Molecular Weight: 32,118 Da
• NCBI Official Full Name: purine nucleoside phosphorylase
• NCBI Official Synonym Full Names: purine nucleoside phosphorylase
• NCBI Official Symbol: PNP
• NCBI Official Synonym Symbols: NP; PUNP; PRO1837
• NCBI Protein Information: purine nucleoside phosphorylase; inosine phosphorylase; inosine-guanosine phosphorylase; purine-nucleoside:orthophosphate ribosyltransferase
• UniProt Protein Name: Purine nucleoside phosphorylase
• Protein Family: Polyribonucleotide nucleotidyltransferase
• UniProt Gene Name: PNP
• UniProt Synonym Gene Names: NP; PNP
• UniProt Entry Name: PNPH_HUMAN

NCBI Description

This gene encodes an enzyme which reversibly catalyzes the phosphorolysis of purine nucleosides. The enzyme is trimeric, containing three identical subunits. Mutations which result in nucleoside phosphorylase deficiency result in defective T-cell (cell-mediated) immunity but can also affect B-cell immunity and antibody responses. Neurologic disorders may also be apparent in patients with immune defects. A known polymorphism at aa position 51 that does not affect enzyme activity has been described. A pseudogene has been identified on chromosome 2. [provided by RefSeq, Jul 2008]

Uniprot Description

NP: a metabolic enzyme with purine-nucleoside phosphorylase activity. Defects are the cause of nucleoside phosphorylase deficiency (NP deficiency), with severe T-cell immunodeficiency with neurologic disorder in children.

Protein type: Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; EC 2.4.2.1; Transferase

Chromosomal Location of Human Ortholog: 14q13.1

Cellular Component: cytoskeleton; cytoplasm; intracellular; cytosol

Molecular Function: purine binding; nucleoside binding; drug binding; phosphate binding; purine-nucleoside phosphorylase activity

Biological Process: response to drug; nicotinamide riboside catabolic process; inosine catabolic process; nucleobase, nucleoside and nucleotide metabolic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; purine nucleotide catabolic process; immune response; positive regulation of T cell proliferation; positive regulation of alpha-beta T cell differentiation; purine base metabolic process; purine salvage

Disease: Purine Nucleoside Phosphorylase Deficiency