PLGF/PIGF2/PGF Active Protein | PLGF active protein

Human CellExp PLGF / PIGF2 / PGF, human recombinant

Cat. Num. AAA844157

• Gene Names: PGF; PGFL; PLGF; PlGF-2; D12S1900; SHGC-10760
• Applications: SDS-Page
• Purity: >=95%
• Synonyms: PLGF/PIGF2/PGF; Human CellExp PLGF / PIGF2 / PGF; human recombinant; PGF; PLGF; PlGF2; PlGF; PGFL; SHGC-10760; PLGF active protein

• Host: HEK 293 cells
• Purity/Purification: >=95%
• Form/Format: Lyophilized from 0.22 mum filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.; Appearance: Lyophilized powder
• Sequence Length: 149

• Applicable Applications for PLGF active protein: SDS-PAGE; HPLC
• Solubility/Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 ug/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 month. For extended storage, it is recommended to store at -20 degree C.
• Endotoxin Level: < 0.1 ng/mug of protein (<1EU/mug).
• Activity (Specifications/test method): Measured in a cell proliferation assay using MDA - MB - 231 human breast cancer cells. The ED50 for this effect is typically 1 - 10 ug/ml.
• Biological Activity: Measured in a cell proliferation assay using MDA - MB - 231 human breast cancer cells. The ED50 for this effect is typically 1 - 10 ug/ml.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for PLGF active protein

Background: Placental growth factor (PGF) also known as vascular endothelial growth factor-related protein, PLGF and PlGF2, is a member of the VEGF (vascular endothelial growth factor) sub-family - a key molecule in angiogenesis and vasculogenesis, in particular during embryogenesis. The main source of PGF during pregnancy is the placental trophoblast. PGF is also expressed in many other tissues, including the villous trophoblast. PGF is actived in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. PlGF2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. Also promotes cell tumor growth.

Product Categories/Family for PLGF active protein

Cytokines; Growth Factors & Hormones; Human cell expressed recombinant proteinsProteins and Enzymes; Human cell expressed recombinant proteins Cytokines; Growth Factors & Hormones; VEGFsCytokines; Growth Factors & Hormones; Cytokines & Growth Factors (A-Z

NCBI and Uniprot Product Information

• NCBI GI #: 333033785
• NCBI GeneID: 5228
• NCBI Accession #: NP_001193941.1
• NCBI GenBank Nucleotide #: NM_001207012.1
• UniProt Accession #: P49763; Q07101; Q9BV78; Q9Y6S8
• Molecular Weight: The protein is fused with 6×His tag at the C-terminus, has a calculated MW of 20.2 kDa. The predicted N-terminus is Leu 19. DTT-reduced Protein migrates as 28-33 kDa due to glycosylation.
• NCBI Official Full Name: placenta growth factor isoform 2
• NCBI Official Synonym Full Names: placental growth factor
• NCBI Official Symbol: PGF
• NCBI Official Synonym Symbols: PGFL; PLGF; PlGF-2; D12S1900; SHGC-10760
• NCBI Protein Information: placenta growth factor
• UniProt Protein Name: Placenta growth factor
• UniProt Gene Name: PGF
• UniProt Synonym Gene Names: PGFL; PLGF; PlGF
• UniProt Entry Name: PLGF_HUMAN

NCBI Description

This gene encodes a growth factor found in placenta which is homologous to vascular endothelial growth factor. Alternatively spliced transcripts encoding different isoforms have been found for this gene.[provided by RefSeq, Jun 2011]

Uniprot Description

PGF: Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. Also promotes cell tumor growth. Belongs to the PDGF/VEGF growth factor family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 14q24.3

Cellular Component: extracellular space; membrane; extracellular region

Molecular Function: heparin binding; protein binding; protein homodimerization activity; growth factor activity; protein heterodimerization activity

Biological Process: response to drug; organ regeneration; female pregnancy; signal transduction; cellular response to hormone stimulus; positive regulation of angiogenesis; cell-cell signaling; ureteric bud branching; positive regulation of cell division; positive regulation of cell proliferation; response to hypoxia; positive regulation of endothelial cell proliferation; sprouting angiogenesis; cell differentiation; vascular endothelial growth factor receptor signaling pathway