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Testing Data

Rabbit FoxH1 Polyclonal Antibody | anti-FoxH1 antibody

FoxH1 Antibody

Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity purified
Synonyms
FoxH1; Polyclonal Antibody; FoxH1 Antibody; FOX H1; FOXH 1; Forkhead box H1; Forkhead box protein H1; Forkhead activin signal transducer 1; Forkhead activin Signal Transducer 2; FAST-1; FAST1; anti-FoxH1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purified
Form/Format
100 ug (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
Appearance: Colorless liquid
Concentration
0.5 mg/ml (varies by lot)
Sequence Length
401
Applicable Applications for anti-FoxH1 antibody
Western Blot (WB)
Application Notes
Western blotting (0.5-4 ug/ml). However, the optimal conditions should be determined individually. The antibody recognizes ~44kDa of FoxH1 from samples of human, mouse and rat origins. Reactivity to other species has not been tested.
Immunogen
Synthetic peptide surrounding amino acid 55 of mouse FoxH1
Handling
The antibody solution should be gently mixed before use.
Preparation and Storage
At -20 degree C
Shelf Life: 12 months

Testing Data

Testing Data
Related Product Information for anti-FoxH1 antibody
Background: FoxH1 (also known as Forkhead box H1 or FAST-1) is a transcription factor that shares close homology with Xenopus FAST-1. FoxH1 binds to SMAD2/4 and activates activin response thromugh a DNA binding motif region that consist of TGT(G/T)(T/G)ATT sequence. FoxH1 binding to Smad2/Smad4/FAST1 forms a transcriptionally complex structure that can induced TGFbeta signal transduction.

NCBI and Uniprot Product Information

NCBI GI #
Molecular Weight
43,490 Da
NCBI Official Full Name
forkhead activin signal transducer 2
UniProt Protein Name
Forkhead box protein H1
Protein Family
UniProt Gene Name
Foxh1
UniProt Synonym Gene Names
Fast1; Fast2; Fast-1; Fast-2
UniProt Entry Name
FOXH1_MOUSE

Uniprot Description

FOXH1: Transcriptional activator. Recognizes and binds to the DNA sequence 5'-TGT[GT][GT]ATT-3'. Required for induction of the goosecoid (GSC) promoter by TGF-beta or activin signaling. Forms a transcriptionally active complex containing FOXH1/SMAD2/SMAD4 on a site on the GSC promoter called TARE (TGF-beta/activin response element).

Protein type: DNA-binding; Nuclear receptor co-regulator; Transcription factor

Cellular Component: transcription factor complex; nuclear chromatin; nucleus

Molecular Function: protein domain specific binding; protein binding; DNA binding; androgen receptor binding; sequence-specific DNA binding; bHLH transcription factor binding; SMAD binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; transcription, DNA-dependent; embryonic heart tube anterior/posterior pattern formation; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; negative regulation of estrogen receptor signaling pathway; regulation of transcription from RNA polymerase II promoter; anterior/posterior pattern formation; regulation of transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; axial mesoderm development; positive regulation of transcription from RNA polymerase II promoter; heart looping; determination of left/right symmetry

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Product Notes

The FoxH1 foxh1 (Catalog #AAA840650) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FoxH1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's FoxH1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western blotting (0.5-4 ug/ml). However, the optimal conditions should be determined individually. The antibody recognizes ~44kDa of FoxH1 from samples of human, mouse and rat origins. Reactivity to other species has not been tested. Researchers should empirically determine the suitability of the FoxH1 foxh1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FoxH1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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