Rabbit TEM-8/ATR1 Polyclonal Antibody | anti-TEM-8/ATR1 antibody

TEM-8 / ATR1 Antibody

Cat. Num. AAA840591

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity purified
• Synonyms: TEM-8/ATR1; Polyclonal Antibody; TEM-8 / ATR1 Antibody; Tumor Endothelial Marker 8; TEM-8; TEM8; ATR; FLJ10601; FLJ11298; FLJ21776; anti-TEM-8/ATR1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: 100 ug (0.2mg/ml) affinity purified rabbit anti-TEM8/ATR1 polyclonal antibody in phosphate-buffered saline (PBS) containing 0.5% BSA, 30% glycerol, and 0.01% thimerosal.; Appearance: Colorless liquid
• Concentration: 0.2 mg/ml (varies by lot)
• Sequence Length: 562

• Applicable Applications for anti-TEM-8/ATR1 antibody: Western Blot (WB)
• Application Notes: Western blotting (0.5-4 ug/ml). However, the optimal concentrations should be determined individually. The antibody recognizes ~80-85 kDa doublet of TME8 in samples from human, mouse and rat origins. Reactivity to other species has not been tested.
• Immunogen: Synthetic peptide corresponding to residues surrounding amino acids 484 of Mouse TEM8
• Handling: The antibody solution should be gently mixed before use.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for anti-TEM-8/ATR1 antibody

Background: Tumor Endothelial Markers (TEMs, TEM 1-9) are recently identified genes, whose expression is elevated in tumor-associated endothelium. TEMs are barely detectable in normal endothelial cells. TEM8 has been shown to interact with C5 domain of collagen a3(VI), a protein also preferentially expressed in tumor endothelium. It is thomught that TEM8 may play a role in tumor angiogenesis.

NCBI and Uniprot Product Information

• NCBI GI #: 15987505
• Molecular Weight: 58,615 Da
• NCBI Official Full Name: tumor endothelial marker 8
• UniProt Protein Name: Anthrax toxin receptor 1
• UniProt Gene Name: Antxr1
• UniProt Synonym Gene Names: Atr; Tem8
• UniProt Entry Name: ANTR1_MOUSE

Uniprot Description

ANTXR1: Plays a role in cell attachment and migration. Interacts with extracellular matrix proteins and with the actin cytoskeleton. Mediates adhesion of cells to type 1 collagen and gelatin, reorganization of the actin cytoskeleton and promotes cell spreading. Plays a role in the angiogenic response of cultured umbilical vein endothelial cells. Defects in ANTXR1 are associated with susceptibility to hemangioma capillary infantile (HCI). HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma. Belongs to the ATR family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Actin-binding; Membrane protein, integral; Receptor, misc.

Cellular Component: filopodium membrane; cell surface; cell projection; membrane; integral to membrane; plasma membrane

Molecular Function: actin filament binding; collagen binding; transmembrane receptor activity; metal ion binding; receptor activity

Biological Process: actin cytoskeleton reorganization; reproductive process; signal transduction