Rabbit anti-Human RGS3 Polyclonal Antibody | anti-RGS3 antibody

RGS3 Antibody

Cat. Num. AAA840352

• Gene Names: RGS3; C2PA; RGP3
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation
• Purity: Affinity purified
• Synonyms: RGS3; Polyclonal Antibody; RGS3 Antibody; 602189; P49796; C2PA; FLJ20370; FLJ31516; FLJ90496; PDZ-RGS3; RGP3; anti-RGS3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: 100 ug (0.2 mg/ml) protein A-purified rabbit anti-RGS3 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% sodium azide.; Appearance: Colorless liquid
• Concentration: 0.2 mg/ml (varies by lot)
• Sequence Length: 519

• Applicable Applications for anti-RGS3 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Western blot analysis (1 ug/ml) and immunoprecipitation. However, the optimal conditions should be determined individually.
• Immunogen: E. coli-expressed human RGS3
• Handling: The antibody solution should be gently mixed before use.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for anti-RGS3 antibody

Background: RGS3 (regulator of G protein signaling 3) is a 519-residue protein that is widely expressed in many tissues and cells. The protein contains an RGS domain near the carboxy terminus that serves as a GTPase-activating protein (GAP). RGS3 negatively regulates the activation of Gialpha and Galphaq. A truncated RGS3 (RGS3T), with its N-terminal 313 residues removed, has been shown to negatively regulate acenylyl cyclase and PLC. RGS3T may be localized in the nucleus and RGS3 is found in cytoplasm. Translocation to membrane is important for the RGS proteins to interact with heterotrimeric G proteins.

NCBI and Uniprot Product Information

• NCBI GI #: 442654297
• NCBI GeneID: 5998
• NCBI Accession #: NP_001263189.1
• NCBI GenBank Nucleotide #: NM_001276260.1
• UniProt Accession #: P49796; Q5VXB8; Q5VXC1; Q5VZ05; Q5VZ06; Q6ZRM5; Q8IUQ1; Q8NC47; Q8NFN4; A6NHA0; A8K0V1; B3KUB2
• Molecular Weight: 66,478 Da
• NCBI Official Full Name: regulator of G-protein signaling 3 isoform 2
• NCBI Official Synonym Full Names: regulator of G-protein signaling 3
• NCBI Official Symbol: RGS3
• NCBI Official Synonym Symbols: C2PA; RGP3
• NCBI Protein Information: regulator of G-protein signaling 3
• UniProt Protein Name: Regulator of G-protein signaling 3
• Protein Family: Regulator of G-protein signaling
• UniProt Gene Name: RGS3
• UniProt Synonym Gene Names: RGP3; RGS3
• UniProt Entry Name: RGS3_HUMAN

NCBI Description

This gene encodes a member of the regulator of G-protein signaling (RGS) family. This protein is a GTPase-activating protein that inhibits G-protein-mediated signal transduction. Alternative splicing and the use of alternative promoters results in multiple transcript variants encoding different isoforms. Long isoforms are largely cytosolic and plasma membrane-associated with a function in Wnt signaling and in the epithelial mesenchymal transition, while shorter N-terminally-truncated isoforms can be nuclear. [provided by RefSeq, Jan 2013]

Uniprot Description

RGS3: Down-regulates signaling from heterotrimeric G-proteins by increasing the GTPase activity of the alpha subunits, thereby driving them into their inactive GDP-bound form. Down-regulates G- protein-mediated release of inositol phosphates and activation of MAP kinases. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: GAPs, RGS; GAPs

Chromosomal Location of Human Ortholog: 9q32

Cellular Component: nucleoplasm; cytoplasm; plasma membrane; cytosol

Molecular Function: GTPase activator activity

Biological Process: regulation of G-protein coupled receptor protein signaling pathway; inactivation of MAPK activity; positive regulation of GTPase activity