Mouse PSMC3 Monoclonal Antibody | anti-PSMC3 antibody

PSMC3 antibody

Cat. Num. AAA838189

• Gene Names: PSMC3; TBP1
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: PSMC3 antibody was purified by affinity chromatography.
• Synonyms: PSMC3; Monoclonal Antibody; PSMC3 antibody; Monoclonal PSMC3; Anti-PSMC3; Proteasome 26S subunit ATPase 3; TBP1; Proteasome subunit P50; Tat-binding protein 1; MGC8487; Human immunodeficiency virus tat transactivator binding protein 1; PSMC 3; PSMC-3; anti-PSMC3 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 9C11
• Purity/Purification: PSMC3 antibody was purified by affinity chromatography.
• Form/Format: Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 500 ug-1 mg/ml (varies by lot)
• Sequence Length: 404

• Applicable Applications for anti-PSMC3 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC: 1:100; IF: 1:100; IHC: 1:50; WB: 1:2000
• Biological Significance: The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composedof 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in anATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases that have chaperone-like activity.
• Cross-Reactivity: Human
• Immunogen: PSMC3 antibody was raised in mouse using a full length recombinant protein of human PSMC3 (NP_002795) produced in HEK293T cells, as the immunogen.
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of HEK293T cells transfected with either recombinant PSMC3 protein (red) or empty vector (blue) stained using PSMC3 antibody)

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant PSMC3 protein (Right) or empty vector (Left) detected with PSMC3 antibody)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of PSMC3 protein in paraffin embedded Carcinoma of Human liver tissue using PSMC3 antibody)

Immunofluorescence (IF)

(Immunofluorescent staining of COS7 cells transiently transfected with recombinant PSMC3 protein using PSMC3 antibody)

Related Product Information for anti-PSMC3 antibody

Mouse monoclonal PSMC3 antibody

Product Categories/Family for anti-PSMC3 antibody

Protein Modification & Stress Response

NCBI and Uniprot Product Information

• NCBI GI #: 48145579
• NCBI GeneID: 5702
• UniProt Accession #: P17980; Q3B757; Q3B865; Q53HU5; Q6GPG8; Q6IBS1; Q96HD3; B2R8V1
• Molecular Weight: 49,204 Da
• NCBI Official Full Name: PSMC3
• NCBI Official Synonym Full Names: proteasome (prosome, macropain) 26S subunit, ATPase, 3
• NCBI Official Symbol: PSMC3
• NCBI Official Synonym Symbols: TBP1
• NCBI Protein Information: 26S protease regulatory subunit 6A
• UniProt Protein Name: 26S protease regulatory subunit 6A
• Protein Family: 26S protease regulatory
• UniProt Gene Name: PSMC3
• UniProt Synonym Gene Names: TBP1; TBP-1
• UniProt Entry Name: PRS6A_HUMAN

NCBI Description

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases that have chaperone-like activity. This subunit may compete with PSMC2 for binding to the HIV tat protein to regulate the interaction between the viral protein and the transcription complex. A pseudogene has been identified on chromosome 9. [provided by RefSeq, Jul 2008]

Uniprot Description

RPT5: The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex. In case of HIV-1 infection, suppresses Tat-mediated transactivation. Belongs to the AAA ATPase family.

Protein type: Proteasome complex; Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: nucleoplasm; proteasome complex; membrane; perinuclear region of cytoplasm; nucleus; cytosol

Molecular Function: protein binding; transcription coactivator activity; ATPase activity; transcription corepressor activity; ATP binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; blastocyst development; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; negative regulation of apoptosis; G1/S transition of mitotic cell cycle