Human Apolipoprotein A-V ELISA Kit | APOA5 elisa kit

Human Apolipoprotein A-V ELISA Kit

Cat. Num. AAA2880289

• Gene Names: APOA5; RAP3; APOAV
• Reactivity: Human
• Synonyms: Apolipoprotein A-V; Human Apolipoprotein A-V ELISA Kit; Apo-AV; ApoA-V; Apolipoprotein A5; Regeneration-associated protein 3; APOA5; RAP3; UNQ411/PRO773; APOA5 elisa kit

• Reactivity: Human
• Specificity: Recombinant and natural Human Arginine decarboxylase,ADC
• Sequence Length: 366

• Samples: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates Or Other Biological Fluids
• Detection Range: 0.78-50 ng/mL
• Sensitivity: <0.36 ng/mL
• Intra-assay Precision: Intra-Assay CV: <=4.5%
• Inter-assay Precision: Inter-Assay CV: <=7.6%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for APOA5 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 262231737
• NCBI GeneID: 116519
• NCBI Accession #: NP_001160070.1
• NCBI GenBank Nucleotide #: NM_001166598.1
• UniProt Accession #: Q6Q788; Q3MIK6; Q6UWK9; Q9UBJ3; B0YIV9
• Molecular Weight: 41,213 Da
• NCBI Official Full Name: apolipoprotein A-V
• NCBI Official Synonym Full Names: apolipoprotein A-V
• NCBI Official Symbol: APOA5
• NCBI Official Synonym Symbols: RAP3; APOAV
• NCBI Protein Information: apolipoprotein A-V
• UniProt Protein Name: Apolipoprotein A-V
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOA5
• UniProt Synonym Gene Names: RAP3; Apo-AV; ApoA-V
• UniProt Entry Name: APOA5_HUMAN

NCBI Description

The protein encoded by this gene is an apolipoprotein that plays an important role in regulating the plasma triglyceride levels, a major risk factor for coronary artery disease. It is a component of high density lipoprotein and is highly similar to a rat protein that is upregulated in response to liver injury. Mutations in this gene have been associated with hypertriglyceridemia and hyperlipoproteinemia type 5. This gene is located proximal to the apolipoprotein gene cluster on chromosome 11q23. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, Oct 2009]

Uniprot Description

APOA5: Minor apolipoprotein mainly associated with HDL and to a lesser extent with VLDL. May also be associated with chylomicrons. Important determinant of plasma triglyceride (TG) levels by both being a potent stimulator of apo-CII lipoprotein lipase (LPL) TG hydrolysis and a inhibitor of the hepatic VLDL-TG production rate (without affecting the VLDL-apoB production rate). Activates poorly lecithin:cholesterol acyltransferase (LCAT) and does not enhance efflux of cholesterol from macrophages. Interacts with GPIHBP1. Up-regulated by PPARA agonists, which are used clinically to lower serum TG (such as fibrates). Liver and plasma. Belongs to the apolipoprotein A1/A4/E family.

Protein type: Secreted; Secreted, signal peptide; Lipid-binding

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: chylomicron; extracellular region; extracellular space

Molecular Function: cholesterol binding; cholesterol transporter activity; enzyme activator activity; enzyme binding; heparin binding; lipid binding; low-density lipoprotein receptor binding; phosphatidylcholine binding; phospholipid binding

Biological Process: acylglycerol homeostasis; cellular lipid metabolic process; cholesterol biosynthetic process; cholesterol efflux; cholesterol homeostasis; lipid transport; lipoprotein metabolic process; neurite regeneration; phosphatidylcholine metabolic process; phospholipid efflux; positive regulation of fatty acid biosynthetic process; positive regulation of lipid catabolic process; positive regulation of lipoprotein lipase activity; positive regulation of receptor-mediated endocytosis; regulation of cholesterol absorption; reverse cholesterol transport; tissue regeneration; triacylglycerol catabolic process; triacylglycerol metabolic process

Disease: Hyperlipoproteinemia, Type V; Hypertriglyceridemia, Familial