Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded SCC25 xenograft using ZPBP antibody at a dilution of 1:100)
Rabbit ZPBP Polyclonal Antibody | anti-ZPBP antibody
ZPBP antibody
Applications
Immunohistochemistry, Western Blot
Purity
ZPBP antibody was purified by antigen-affinity chromatography
Synonyms
ZPBP; Polyclonal Antibody; ZPBP antibody; Polyclonal ZPBP; Anti-ZPBP; ZPBP1; zona pellucida binding protein 1; zona pellucida binding protein; FLJ93221; zona pellucida-binding protein 1; anti-ZPBP antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
ZPBP antibody was purified by antigen-affinity chromatography
Form/Format
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
0.48 mg/ml (varies by lot)
Applicable Applications for anti-ZPBP antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
IHC-P: 1:100-1:500
WB: 1:500-1:3000
WB: 1:500-1:3000
Biological Significance
ZPBP is one of several proteins that are thought to participate in secondary binding between acrosome-reacted sperm and the egg-specific extracellular matrix, the zona pellucida.
Cross-Reactivity
Human
Immunogen
ZPBP antibody was raised in rabbit using a recombinant protein fragment contain a sequence corresponding to a region within amino acids 29 and 275 of ZPBP
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded SCC25 xenograft using ZPBP antibody at a dilution of 1:100)
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded SCC25 xenograft using ZPBP antibody at a dilution of 1:100)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A:A431; B:HeLa S3) using a 12 % SDS PAGE gel and ZPBP antibody at a dilution of 1:500)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A:A431; B:HeLa S3) using a 12 % SDS PAGE gel and ZPBP antibody at a dilution of 1:500)
Related Product Information for anti-ZPBP antibody
Affinity purified Rabbit polyclonal ZPBP antibody
Product Categories/Family for anti-ZPBP antibody
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Product Notes
The ZPBP (Catalog #AAA837580) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's ZPBP can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IHC-P: 1:100-1:500 WB: 1:500-1:3000. Researchers should empirically determine the suitability of the ZPBP for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZPBP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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