SDS-PAGE
(Figure annotation denotes ug of protein loaded and % gel used.)
Glyoxalase I Active Protein | Arad_1397 active protein
Glyoxalase I protein
Applications
SDS-Page
Purity
> 90% pure
Synonyms
Glyoxalase I; Glyoxalase I protein; GLO-1 Methylglyoxalase protein; Lactoylglutathione lyase protein; Lactoyl glutathione lyase protein; Ketone aldehyde mutase protein; Glx 1 protein; GLOD1 protein; S D lactoylglutathione methylglyoxal lyase 0.; GLYI protein; Glx I protein; Aldoketomutase protein; Arad_1397 active protein
Host
Human
Purity/Purification
> 90% pure
Form/Format
Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 1mM DTT, and 10% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence
1-184 amino acids: MAEPQPPSGG LTDEAALSCC SDADPSTKDF LLQQTMLRVK DPKKSLDFYT RVLGMTLIQK CDFPIMKFSL YFLAYEDKND IPKEKDEKIA WALSRKATLE LTHNWGTEDD ETQSYHNGNS DPRGFGHIGI AVPDVYSACK RFEELGVKFV KKPDDGKMKG LAFIQDPDGY WIEILNPNKM ATLM
Sequence Length
259
Applicable Applications for Arad_1397 active protein
SDS-PAGE
Application Notes
1. Prepare a 1.5ml reaction mix into a suitable container: The final concentrations are 79 mM potassium phosphate, 0.033%(w/v) reduced glutathine, 0.003% (w/v) bovine serum albumin. 2. Equilibrate to 25 DEG C and monitor at A240nm (absorbance) until the value is constant, using a spectrophotometer. 3. Add 50ul of recombinant glyoxalase I solution with various concentrations (0.5ug, 1ug, 2ug) in 1.4ml reaction buffer. 4. Immediately mix by inversion and record the increase at A240nm for 5 minutes.
Protein Type
Recombinant
Biological Significance
Glyoxalase I, also known as GLO1, belongs to the glyoxalase family. Glyoxalase I is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutathione.This enzyme is ubiquitously expressed and is also present in many tumor cell lines, in which its concentration is often upregulated. Recombinant human GLO1 protein was expressed in E Coli and purified by using conventional chromatography techniques.
Bioactivity
Specific activity: >0.4 units/mg (please enquire for specific batch value). One unit will form 1.0 umol of S-lactoylglutathione from methylglyoxal and reduced glutathione per minute at pH 7.5, at 25 deg C.
Expression System
E Coli
Preparation and Storage
Store at 4 degree C for short term storage (1/2 weeks). Aliquot and store at -20 degree C or - 70 degree C for long term storage. Avoid repeated freeze/thaw cycles.
SDS-PAGE
(Figure annotation denotes ug of protein loaded and % gel used.)
SDS-PAGE
(Figure annotation denotes ug of protein loaded and % gel used.)
Related Product Information for Arad_1397 active protein
Purified recombinant Human Glyoxalase I protein
Product Categories/Family for Arad_1397 active protein
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
20.7 kDa (184 aa), confirmed by MALDI-TOF.
NCBI Official Full Name
glyoxalase protein
UniProt Protein Name
Glyoxalase protein
UniProt Gene Name
Arad_1397
UniProt Entry Name
B9JBE1_AGRRK
Similar Products
Product Notes
The Glyoxalase I arad_1397 (Catalog #AAA837132) is an Active Protein produced from Human and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Glyoxalase I can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. 1. Prepare a 1.5ml reaction mix into a suitable container: The final concentrations are 79 mM potassium phosphate, 0.033%(w/v) reduced glutathine, 0.003% (w/v) bovine serum albumin. 2. Equilibrate to 25 DEG C and monitor at A240nm (absorbance) until the value is constant, using a spectrophotometer. 3. Add 50ul of recombinant glyoxalase I solution with various concentrations (0.5ug, 1ug, 2ug) in 1.4ml reaction buffer. 4. Immediately mix by inversion and record the increase at A240nm for 5 minutes. Researchers should empirically determine the suitability of the Glyoxalase I arad_1397 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: 1-184 amino acids: MAEPQPPSGG LTDEAALSCC SDADPSTKDF LLQQTMLRVK DPKKSLDFYT RVLGMTLIQK CDFPIMKFSL YFLAYEDKND IPKEKDEKIA WALSRKATLE LTHNWGTEDD ETQSYHNGNS DPRGFGHIGI AVPDVYSACK RFEELGVKFV KKPDDGKMKG LAFIQDPDGY WIEILNPNKM ATLM. It is sometimes possible for the material contained within the vial of "Glyoxalase I, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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