14.3.3 eta recombinant protein

14.3.3 eta protein (His tag)

Cat. Num. AAA836162

• Gene Names: YWHAH; YWHA1
• Applications: SDS-Page
• Purity: > 95% pure
• Synonyms: 14.3.3 eta; 14.3.3 eta protein (His tag); Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein protein; Tyrosine 3/tryptophan 5 monooxygenase activation protein eta polypeptide protein; Protein AS1 protein; YWHAH protein; YWHA 1.; HGNC:12853 protein; YWHA1 protein; 14-3-3 eta protein; eta polypeptide 14 3 3 protein eta protein; 14-3-3 eta; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein eta polypeptide protein; eta polypeptide protein; 14/3/3 eta protein; 14/3/3 eta; 14.3.3 eta recombinant protein

• Host: E. coli
• Purity/Purification: > 95% pure
• Form/Format: Supplied as a liquid in 20mM Tris, pH 8.0. Has a His-tag
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence: 1-246 amino acids: MGSSHHHHHH SSGLVPRGSH MGDREQLLQR ARLAEQAERY DDMASAMKAV TELNEPLSNE DRNLLSVAYK NVVGARRSSW RVISSIEQKT MADGNEKKLE KVKAYREKIE KELETVCNDV LSLLDKFLIK NCNDFQYESK VFYLKMKGDY YRYLAEVASG EKKNSVVEAS EAAYKEAFEI SKEQMQPTHP IRLGLALNFS VFYYEIQNAP EQACLLAKQA FDDAIAELDT LNEDSYKDST LIMQLLRDNL TLWTSDQQDE EAGEGN
• Sequence Length: 246

• Applicable Applications for 14.3.3 eta recombinant protein: SDS-PAGE
• Application Notes: 14.3.3 eta protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
• Grade: Recombinant
• Biological Significance: The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon,sigma, zeta, tau and eta that have been identified in mammals. The 14-3-3eta (eta), a subtype of the 14-3-3 family of proteins, was found in B cells, brain, cerebrospinal fluid etc. 14-3-3eta interacts with and relocalizes the A20 zinc finger protein from the insoluble to the soluble fraction, suggesting a chaperone function. Recombinant human 14-3-3eta, fused to His-tag at N-terminus, was expressed in E Coli and purified by conventional chromatography techniques.
• Expression System: E Coli
• Tag/Conjugate: His tag
• Biohazard: Not hazardous. Use normal laboratory precautions when handling.
• Preparation and Storage: Store at 4°C for short term.; Long term: -20°C

SDS-PAGE

Related Product Information for 14.3.3 eta recombinant protein

Purified recombinant Human 14.3.3 eta protein

Product Categories/Family for 14.3.3 eta recombinant protein

Signal Transduction; Conjugated Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 4507951
• NCBI GeneID: 7533
• NCBI Accession #: NP_003396.1
• NCBI GenBank Nucleotide #: NM_003405.3
• UniProt Accession #: Q04917
• Molecular Weight: 30.3 kDa (266 aa)
• NCBI Official Full Name: 14-3-3 protein eta
• NCBI Official Synonym Full Names: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta
• NCBI Official Symbol: YWHAH
• NCBI Official Synonym Symbols: YWHA1
• NCBI Protein Information: 14-3-3 protein eta
• UniProt Protein Name: 14-3-3 protein eta
• UniProt Gene Name: YWHAH
• UniProt Synonym Gene Names: YWHA1
• UniProt Entry Name: 1433F_HUMAN

NCBI Description

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and bovine orthologs. This gene contains a 7 bp repeat sequence in its 5' UTR, and changes in the number of this repeat have been associated with early-onset schizophrenia and psychotic bipolar disorder. [provided by RefSeq, Jun 2009]

Uniprot Description

14-3-3 eta: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes.

Protein type: Adaptor/scaffold; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 22q12.3

Cellular Component: cytoplasmic vesicle membrane; cytoplasm; plasma membrane; cytosol

Molecular Function: insulin-like growth factor receptor binding; protein domain specific binding; protein binding; enzyme binding; sodium channel regulator activity; protein heterodimerization activity; actin binding; glucocorticoid receptor binding

Biological Process: intracellular protein transport; substantia nigra development; apoptosis; regulation of neuron differentiation; positive regulation of transcription, DNA-dependent; glucocorticoid receptor signaling pathway; regulation of synaptic plasticity; glucocorticoid catabolic process; negative regulation of dendrite morphogenesis; regulation of sodium ion transport