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Western Blot (WB) (Western blot analysis of Syndecan-4 with Jurkat cell lysate.)

Rabbit Syndecan 4 Polyclonal Antibody | anti-SDC4 antibody

Syndecan 4 antibody

Gene Names
SDC4; SYND4
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Syndecan 4 antibody was purified by affinity chromatography
Synonyms
Syndecan 4; Polyclonal Antibody; Syndecan 4 antibody; Polyclonal Syndecan 4; anti-Syndecan 4; SDC4; Syndecan4; Syndecan -4; SYND4; Syndecan-4; anti-SDC4 antibody
Ordering
For Research Use Only!
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Syndecan 4 antibody was purified by affinity chromatography
Form/Format
Supplied in PBS buffer, pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal
Concentration
500 ug/ml (varies by lot)
Sequence Length
198
Applicable Applications for anti-SDC4 antibody
Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
IP: 5-10 ug/ml
IHC: 15-25 ug/ml
WB: 0.5-4 ug/ml
Biological Significance
Syndecans are type I integral membrane proteoglycans that are involved in cell-extracellular matrix adhesion and growth factor binding. Syndecan-1 is an matrix receptor which binds to Collagens, Fibronectin and Thrombospondin. Syndecan-1 and Syndecan-3 interact with MK, a growth/differentiation factor involved in embryogenesis. Syndecan-2 is highly expressed at areas of high morphogenetic activity, such as epithelial-mesenchymal interfaces and the prechondrogenic and preosteogenic mesenchymal condensations. Syndecan-4 functions cooperatively with integrins in the processes of cell spreading and focal adhesion assembly.
Cross-Reactivity
Human, Mouse, Rat
Immunogen
Syndecan 4 antibody was raised in Rabbit using synthetic peptide surrounding amino acid 184 of human Syndecan-4 as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of Syndecan-4 with Jurkat cell lysate.)

Western Blot (WB) (Western blot analysis of Syndecan-4 with Jurkat cell lysate.)
Related Product Information for anti-SDC4 antibody
Rabbit polyclonal Syndecan 4 antibody
Product Categories/Family for anti-SDC4 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
16,545 Da
NCBI Official Full Name
Syndecan 4
NCBI Official Synonym Full Names
syndecan 4
NCBI Official Symbol
SDC4
NCBI Official Synonym Symbols
SYND4
NCBI Protein Information
syndecan-4
UniProt Protein Name
Syndecan-4
Protein Family
UniProt Gene Name
SDC4
UniProt Synonym Gene Names
SYND4
UniProt Entry Name
SDC4_HUMAN

NCBI Description

The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan that functions as a receptor in intracellular signaling. The encoded protein is found as a homodimer and is a member of the syndecan proteoglycan family. This gene is found on chromosome 20, while a pseudogene has been found on chromosome 22. [provided by RefSeq, Jul 2008]

Uniprot Description

syndecan-4: a heparan sulfate proteoglycan type I membrane protein that belongs to the syndecan proteoglycan family. Expressed as a homodimer. Promotes cell spreading in a beta(1) integrin-dependent fashion through PKC-alpha and Rho.

Protein type: Membrane protein, integral; Cell adhesion; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 20q12

Cellular Component: lysosomal lumen; focal adhesion; cell surface; costamere; integral to plasma membrane; Golgi lumen; plasma membrane; lipid raft

Molecular Function: protein binding; protein kinase C binding; fibronectin binding

Biological Process: phototransduction, visible light; extracellular matrix organization and biogenesis; glycosaminoglycan metabolic process; pathogenesis; chondroitin sulfate metabolic process; glycosaminoglycan biosynthetic process; glycosaminoglycan catabolic process; positive regulation of focal adhesion formation; ureteric bud development; positive regulation of protein kinase activity; positive regulation of stress fiber formation; carbohydrate metabolic process; retinoid metabolic process

Research Articles on SDC4

Similar Products

Product Notes

The SDC4 sdc4 (Catalog #AAA835675) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Syndecan 4 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB). IP: 5-10 ug/ml IHC: 15-25 ug/ml WB: 0.5-4 ug/ml. Researchers should empirically determine the suitability of the SDC4 sdc4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Syndecan 4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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