Mouse NPR3 Monoclonal Antibody | anti-NPR3 antibody

NPR3 antibody

Cat. Num. AAA834215

• Gene Names: NPR3; NPRC; ANP-C; ANPRC; NPR-C; ANPR-C; GUCY2B; C5orf23
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: NPR3 antibody was purified by affinity chromatography.
• Synonyms: NPR3; Monoclonal Antibody; NPR3 antibody; Monoclonal NPR3; Anti-NPR3; Natriuretic peptide receptor C; Guanylate cyclase C; Atrionatriuretic peptide receptor C; GUCY2B; ANPR-C; NPR 3; NPR-3; anti-NPR3 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 11B6
• Purity/Purification: NPR3 antibody was purified by affinity chromatography.
• Form/Format: Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 500 ug-1 mg/ml (varies by lot)
• Sequence Length: 540

• Applicable Applications for anti-NPR3 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC: 1:100; IF: 1:100; IHC: 1:50; WB: 1:1000
• Biological Significance: The family of natriuretic peptides elicit a number of vascular, renal, and endocrine effects that are important in the maintenance of blood pressure and extracellular fluid volume. These effects are mediated by specific binding of the peptides to cell surface receptors in the vasculature, kidney, adrenal, and brain.
• Cross-Reactivity: Human
• Immunogen: NPR3 antibody was raised in mouse using a full length recombinant protein of human NPR3 (NP_000899) produced in HEK293T cells, as the immunogen.
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant NPR3 protein (Right) or empty vector (Left) detected with NPR3 antibody)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of NPR3 protein in paraffin embedded Human kidney tissue using NPR3 antibody)

Immunofluorescence (IF)

(Immunofluorescent staining of COS7 cells transiently transfected with recombinant NPR3 protein using NPR3 antibody)

Related Product Information for anti-NPR3 antibody

Mouse monoclonal NPR3 antibody

Product Categories/Family for anti-NPR3 antibody

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 4505441
• NCBI GeneID: 4883
• NCBI Accession #: NP_000899
• NCBI GenBank Nucleotide #: NM_000908.3
• UniProt Accession #: P17342; A2RRD1; B4DT84; E7EPG9
• Molecular Weight: 36,656 Da
• NCBI Official Full Name: atrial natriuretic peptide receptor 3 isoform 2
• NCBI Official Synonym Full Names: natriuretic peptide receptor 3
• NCBI Official Symbol: NPR3
• NCBI Official Synonym Symbols: NPRC; ANP-C; ANPRC; NPR-C; ANPR-C; GUCY2B; C5orf23
• NCBI Protein Information: atrial natriuretic peptide receptor 3
• UniProt Protein Name: Atrial natriuretic peptide receptor 3
• Protein Family: Nitrogen permease regulator
• UniProt Gene Name: NPR3
• UniProt Synonym Gene Names: ANPRC; C5orf23; NPRC; ANP-C; ANPR-C; NPR-C
• UniProt Entry Name: ANPRC_HUMAN

NCBI Description

This gene encodes one of three natriuretic peptide receptors. Natriutetic peptides are small peptides which regulate blood volume and pressure, pulmonary hypertension, and cardiac function as well as some metabolic and growth processes. The product of this gene encodes a natriuretic peptide receptor responsible for clearing circulating and extracellular natriuretic peptides through endocytosis of the receptor. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2011]

Uniprot Description

ANP-C: Receptor for the natriuretic peptide hormones, binding with similar affinities atrial natriuretic peptide NPPA/ANP, brain natriuretic peptide NPPB/BNP, and C-type natriuretic peptide NPPC/CNP. May function as a clearance receptor for NPPA, NPPB and NPPC, regulating their local concentrations and effects. May regulate diuresis, blood pressure and skeletal development. Does not have guanylate cyclase activity. Belongs to the ANF receptor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, GPCR

Chromosomal Location of Human Ortholog: 5p13.3

Cellular Component: integral to plasma membrane

Molecular Function: protein homodimerization activity; hormone binding; natriuretic peptide receptor activity; peptide hormone binding; peptide receptor activity, G-protein coupled

Biological Process: regulation of osteoblast proliferation; phosphoinositide-mediated signaling; negative regulation of smooth muscle cell proliferation; positive regulation of nitric-oxide synthase activity; regulation of blood pressure; negative regulation of adenylate cyclase activity; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); G-protein signaling, adenylate cyclase inhibiting pathway; pancreatic juice secretion; skeletal development