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Western Blot (WB) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ILVBL protein (Right) or empty vector (Left) detected with ILVBL antibody)

Mouse ILVBL Monoclonal Antibody | anti-ILVBL antibody

ILVBL antibody

Gene Names
ILVBL; AHAS; 209L8; ILV2H
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
ILVBL antibody was purified by affinity chromatography.
Synonyms
ILVBL; Monoclonal Antibody; ILVBL antibody; Monoclonal ILVBL; Anti-ILVBL; ilvB bacterial acetolactate synthase like; AHAS; 209L8; ILV2H; IlvB-like protein; anti-ILVBL antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
8A12
Purity/Purification
ILVBL antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
500 ug-1 mg/ml (varies by lot)
Sequence Length
632
Applicable Applications for anti-ILVBL antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
IF: 1:100
FC: 1:100
IHC: 1:150
WB: 1:500-2000
Biological Significance
ILVBL shares similarity with several thiamine pyrophosphate-binding proteins identified inbacteria, yeast, and plants. The highest degree of similarity is found with bacterial acetolactate synthases (AHAS), which are enzymes that catalyze the first step in branched-chain amino acid biosynthesis.
Cross-Reactivity
Human, Rat, Monkey
Immunogen
ILVBL antibody was raised in mouse using a full length recombinant protein of human ILVBL (NP_006835) produced in HEK293T cells, as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ILVBL protein (Right) or empty vector (Left) detected with ILVBL antibody)

Western Blot (WB) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ILVBL protein (Right) or empty vector (Left) detected with ILVBL antibody)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of ILVBL protein in paraffin embedded Adenocarcinoma of Human breast tissue using ILVBL antibody)

Immunohistochemistry (IHC) (Immunohistochemical analysis of ILVBL protein in paraffin embedded Adenocarcinoma of Human breast tissue using ILVBL antibody)

Immunofluorescence (IF)

(Immunofluorescent staining of COS7 cells transiently transfected with recombinant ILVBL protein using ILVBL antibody)

Immunofluorescence (IF) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant ILVBL protein using ILVBL antibody)
Related Product Information for anti-ILVBL antibody
Mouse monoclonal ILVBL antibody
Product Categories/Family for anti-ILVBL antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
67,868 Da
NCBI Official Full Name
acetolactate synthase-like protein
NCBI Official Synonym Full Names
ilvB (bacterial acetolactate synthase)-like
NCBI Official Symbol
ILVBL
NCBI Official Synonym Symbols
AHAS; 209L8; ILV2H
NCBI Protein Information
acetolactate synthase-like protein
UniProt Protein Name
Acetolactate synthase-like protein
UniProt Gene Name
ILVBL
UniProt Synonym Gene Names
AHAS
UniProt Entry Name
ILVBL_HUMAN

NCBI Description

The protein encoded by this gene shares similarity with several thiamine pyrophosphate-binding proteins identified in bacteria, yeast, and plants. The highest degree of similarity is found with bacterial acetolactate synthases (AHAS), which are enzymes that catalyze the first step in branched-chain amino acid biosynthesis. [provided by RefSeq, Jul 2008]

Uniprot Description

ILVBL: shares similarity with several thiamine pyrophosphate-binding proteins identified in bacteria, yeast, and plants. The highest degree of similarity is found with bacterial acetolactate synthases (AHAS), which are enzymes that catalyze the first step in branched-chain amino acid biosynthesis. [provided by RefSeq, Jul 2008]

Protein type: Membrane protein, integral; EC 2.2.1.-; Transferase

Chromosomal Location of Human Ortholog: 19p13.1

Cellular Component: membrane; integral to membrane

Molecular Function: transferase activity; magnesium ion binding; thiamin pyrophosphate binding

Biological Process: metabolic process

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Product Notes

The ILVBL ilvbl (Catalog #AAA834021) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's ILVBL can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB). IF: 1:100 FC: 1:100 IHC: 1:150 WB: 1:500-2000. Researchers should empirically determine the suitability of the ILVBL ilvbl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ILVBL, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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