Mouse RLBP1 Monoclonal Antibody | anti-RLBP1 antibody

RLBP1 antibody

Cat. Num. AAA834005

• Gene Names: RLBP1; CRALBP
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay, Western Blot
• Purity: RLBP1 antibody was purified by affinity chromatography.
• Synonyms: RLBP1; Monoclonal Antibody; RLBP1 antibody; Monoclonal RLBP1; Anti-RLBP1; CRALBP; MGC3663; RLBP 1; RLBP-1; anti-RLBP1 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 1G6
• Purity/Purification: RLBP1 antibody was purified by affinity chromatography.
• Form/Format: Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5-1 mg/ml (varies by lot)
• Sequence Length: 317

• Applicable Applications for anti-RLBP1 antibody: Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Western Blot (WB)
• Application Notes: FC: 1:100; IHC: 1:150; WB: 1:2000
• Biological Significance: The protein encoded by this gene is a 36-kD water-soluble protein which carries 11-cis-retinaldehyde or 11-cis-retinal as physiologic ligands. It may be a functional component of the visual cycle. Mutations of this gene have been associated with severe rod-cone dystrophy, Bothnia dystrophy (nonsyndromic autosomal recessive retinitis pigmentosa) and retinitis punctata albescens.
• Cross-Reactivity: Human
• Immunogen: RLBP1 antibody was raised in Mouse using full length human recombinant protein of human RLBP1 produced in HEK293T cell as the immunogen.
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of HEK293T cells transfected with either recombinant RLBP1 protein (red) or empty vector (blue) stained using RLBP1 antibody)

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant RLBP1 protein (Right) or empty vector (Left) detected with RLBP1 antibody)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of RLBP1 protein in paraffin embedded Carcinoma of Human prostate tissue using RLBP1 antibody)

Related Product Information for anti-RLBP1 antibody

Mouse monoclonal RLBP1 antibody

Product Categories/Family for anti-RLBP1 antibody

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 4506541
• NCBI GeneID: 6017
• NCBI Accession #: NP_000317.1
• NCBI GenBank Nucleotide #: NM_000326.4
• UniProt Accession #: P12271; B2R667
• Molecular Weight: 36,474 Da
• NCBI Official Full Name: retinaldehyde-binding protein 1
• NCBI Official Synonym Full Names: retinaldehyde binding protein 1
• NCBI Official Symbol: RLBP1
• NCBI Official Synonym Symbols: CRALBP
• NCBI Protein Information: retinaldehyde-binding protein 1
• UniProt Protein Name: Retinaldehyde-binding protein 1
• Protein Family: Retinaldehyde-binding protein
• UniProt Gene Name: RLBP1
• UniProt Synonym Gene Names: CRALBP
• UniProt Entry Name: RLBP1_HUMAN

NCBI Description

The protein encoded by this gene is a 36-kD water-soluble protein which carries 11-cis-retinaldehyde or 11-cis-retinal as physiologic ligands. It may be a functional component of the visual cycle. Mutations of this gene have been associated with severe rod-cone dystrophy, Bothnia dystrophy (nonsyndromic autosomal recessive retinitis pigmentosa) and retinitis punctata albescens. [provided by RefSeq, Jul 2008]

Uniprot Description

RLBP1: Soluble retinoid carrier essential the proper function of both rod and cone photoreceptors. Participates in the regeneration of active 11-cis-retinol and 11-cis-retinaldehyde, from the inactive 11-trans products of the rhodopsin photocycle and in the de novo synthesis of these retinoids from 11-trans metabolic precursors. The cycling of retinoids between photoreceptor and adjacent pigment epithelium cells is known as the 'visual cycle'. Defects in RLBP1 are a cause of retinitis pigmentosa autosomal recessive (ARRP). RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. Defects in RLBP1 are the cause of Bothnia retinal dystrophy (BRD); also known as Vasterbotten dystrophy. Affected individuals show night blindness from early childhood with features consistent with retinitis punctata albescens and macular degeneration. Defects in RLBP1 are the cause of rod-cone dystrophy Newfoundland (NFRCD). NFRCD is a retinal dystrophy reminiscent of retinitis punctata albescens but with a substantially lower age at onset and more-rapid and distinctive progression. Rod-cone dystrophies results from initial loss of rod photoreceptors, later followed by cone photoreceptors loss. Defects in RLBP1 are a cause of retinitis punctata albescens (RPA). A rare form of stationary night blindness characterized by a delay in the regeneration of cone and rod photopigments.

Chromosomal Location of Human Ortholog: 15q26

Cellular Component: cytosol

Molecular Function: transporter activity; 11-cis retinal binding; retinol binding

Biological Process: phototransduction, visible light; visual perception; transport; retinoid metabolic process; vitamin A metabolic process

Disease: Fundus Albipunctatus; Bothnia Retinal Dystrophy; Newfoundland Rod-cone Dystrophy