Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant IL36A protein (Right) or empty vector (Left) detected with IL36A antibody)
Mouse IL36A Monoclonal Antibody | anti-IL36A antibody
IL36A antibody
Gene Names
IL36A; FIL1; FIL1E; IL1F6; IL-1F6; IL1(EPSILON); FIL1(EPSILON)
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot
Purity
IL36A antibody was purified by affinity chromatography.
Synonyms
IL36A; Monoclonal Antibody; IL36A antibody; Monoclonal IL36A; Anti-IL36A; FIL1 epsilon; Interleukin 1 epsilon; Interleukin-1 family member 6; IL 36A; IL-36A; anti-IL36A antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3A2
Purity/Purification
IL36A antibody was purified by affinity chromatography.
Form/Format
Liquid in PBS, pH 7.3 with 1% BSA, 50% Glycerol and 0.02% Sodium Azide.
Concentration
1.0 mg/mL (varies by lot)
Sequence Length
158
Applicable Applications for anti-IL36A antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB)
Application Notes
FC: 1: 100
IF: 1:100
WB: 1:200-1: 1000
IF: 1:100
WB: 1:200-1: 1000
Biological Significance
IL36A is a member of the IL-1 family of proteins. Cells reported to express IL-36 alpha include monocytes, B cells, and T cells. Notably, IL-36 alpha is the only novel IL-1 family member expressed on T-cells. The receptor for IL-36 alpha is reported to be a combination of IL-1 R6/IL-1 R rp2 and IL-1 R3/IL-1 R AcP.
Cross-Reactivity
Human
Immunogen
Full length human recombinant protein of human IL1F6 (NP_055255) produced in HEK293T cells.
Preparation and Storage
Store at 4°C for short term, -20°C for long term. Avoid repeat freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant IL36A protein (Right) or empty vector (Left) detected with IL36A antibody)
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant IL36A protein (Right) or empty vector (Left) detected with IL36A antibody)
Immunofluorescence (IF)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant IL36A protein using IL36A antibody)
Immunofluorescence (IF)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant IL36A protein using IL36A antibody)
Flow Cytometry (FC)
Flow Cytometry (FC)
Related Product Information for anti-IL36A antibody
Mouse monoclonal IL36A antibody
Product Categories/Family for anti-IL36A antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
interleukin-36 alpha
NCBI Official Synonym Full Names
interleukin 36, alpha
NCBI Official Symbol
IL36A
NCBI Official Synonym Symbols
FIL1; FIL1E; IL1F6; IL-1F6; IL1(EPSILON); FIL1(EPSILON)
NCBI Protein Information
interleukin-36 alpha
UniProt Protein Name
Interleukin-36 alpha
Protein Family
UniProt Gene Name
IL36A
UniProt Synonym Gene Names
FIL1E; IL1E; IL1F6; IL-1 epsilon; IL-1F6
UniProt Entry Name
IL36A_HUMAN
Uniprot Description
IL1F6: Belongs to the IL-1 family.
Protein type: Cytokine
Chromosomal Location of Human Ortholog: 2q12-q14.1
Cellular Component: extracellular space; extracellular region
Molecular Function: interleukin-1 receptor binding; cytokine activity
Biological Process: cytokine and chemokine mediated signaling pathway; positive regulation of interleukin-6 production; innate immune response; immune response; inflammatory response
Research Articles on IL36A
Similar Products
Product Notes
The IL36A il36a (Catalog #AAA833924) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's IL36A can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB). FC: 1: 100 IF: 1:100 WB: 1:200-1: 1000. Researchers should empirically determine the suitability of the IL36A il36a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL36A, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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