Flow Cytometry (FC/FACS)
(Staining of C57BL/6 splenocytes with NK1.1 antibody and 0.125 ug of CD244.2 antibody (PE). Cells in the lymphocyte gate were used for analysis.)
Rat CD244.2 Monoclonal Antibody | anti-CD244.2 antibody
CD244.2 antibody (PE)
Applications
Flow Cytometry, Functional Assay
Synonyms
CD244.2; Monoclonal Antibody; CD244.2 antibody (PE); Monoclonal CD244.2; Anti-CD244.2; CD-244.2; CD 244.2; B6 Alloantigen; anti-CD244.2 antibody
Host
Rat
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
244F4
Specificity
Mouse
Form/Format
Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration
200 ug/ml (varies by lot)
Applicable Applications for anti-CD244.2 antibody
Flow Cytometry (FC/FACS)
Application Notes
FC: <= 0.5 ug/test
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
Biological Significance
Mouse CD244, also known as 2B4, is a receptor belonging to the CD2 family of proteins involved in non-MHC dependent cytotoxicity. Expression is found on natural killer cells and many NKT cells and CD8+ cells. In C57BL/6 mice, two splice variants exist that differ in the length of the cytoplasmic domain. The long form functions in an inhibitory manner through signaling through SHP-2, while the short form functions in an activating manner. The ligand for CD244 is CD48 which is expressed on all hematopoietic cells. Recent data suggests that CD244 interactions with CD48, but not CD2 interaction with CD48, is essential for IL-2?driven expansion and activation of murine NK cells.Reports show CD244 expression can discriminate hematopoietic progenitors. Hematopoietic stem cells are highly purified as CD150+CD244-CD48- cells while non-self-renewing multipotent hematopoietic progenitors are CD244+CD150-CD48- and the most restricted progenitors are CD48+CD244+CD150-.244F4 was generated against cell lines expressing CD244. It reacts with the C57BL/6 but not Balb/c due to polymorphisms present in the immunoglobulin V domain of the protein. The epitope recognized by 244F4 is different from that of 2B4 therefore these antibodies do not cross-block the binding to the respective epitopes.
Tag/Conjugate
PE
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Protect from light.
Flow Cytometry (FC/FACS)
(Staining of C57BL/6 splenocytes with NK1.1 antibody and 0.125 ug of CD244.2 antibody (PE). Cells in the lymphocyte gate were used for analysis.)
Flow Cytometry (FC/FACS)
(Staining of C57BL/6 splenocytes with NK1.1 antibody and 0.125 ug of CD244.2 antibody (PE). Cells in the lymphocyte gate were used for analysis.)
Related Product Information for anti-CD244.2 antibody
Rat monoclonal CD244.2 antibody (PE)
Product Categories/Family for anti-CD244.2 antibody
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Product Notes
The CD244.2 (Catalog #AAA833816) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD244.2 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC: <= 0.5 ug/test A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Researchers should empirically determine the suitability of the CD244.2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD244.2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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