Immunocytochemistry (ICC)
(O4 in Differentiated Rat Cortical Stem Cells. Oligodendrocyte Marker O4 was detected in immersion fixed differentiated rat cortical stem cells using 1 ug/mL Human/Mouse/Rat/Chicken O4 antibody (MBS832589) for 3 hours at room temperature.)
Mouse O4 Monoclonal Antibody | anti-O4 antibody
O4 antibody
Reactivity
Chicken, Mouse, Rat
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry
Synonyms
O4; Monoclonal Antibody; O4 antibody; Monoclonal O4; Anti-O4; Oligodendrocyte Marker O4; Oligodendrocyte O4; anti-O4 antibody
Host
Mouse
Reactivity
Chicken, Mouse, Rat
Clonality
Monoclonal
Isotype
IgM
Clone Number
O4
Specificity
Detects human, mouse, rat, and chicken Oligodendrocyte Marker O4
Form/Format
Lyophilized from PBS, pH 7.4 with 5% Trehalose. Reconstitute at 0.5 mg/mL in sterile PBS.
Concentration
50 ug, lyophilized (varies by lot)
Applicable Applications for anti-O4 antibody
Flow Cytometry (FC/FACS), Immunohistochemistry (IHC)
Application Notes
FC: 2.5 ug/10^6 cells
IHC: 110 ug/ml
IHC: 110 ug/ml
Biological Significance
Oligodendrocytes and astrocytes are derived from common precursor sells, glioblasts. O-Antigens are sulfatides, which function as differentiation markers on the surface of oligodendrocytes of the central nervous system. O4 is formed postnatallyand is a marker for cell bodies and processes of oligodendrocytes types I and II (hairy eyeball type). During oligodendrocyte differentiation, O4 occurs in prooligodendrocytes, but not in O-2A-progenitor cells. O4 occurs from day 3 onwards in cell cultures of embryonic mouse brain.
Cross-Reactivity
Human, Mouse, Rat, Chicken
Immunogen
Bovine brain corpus callosum white matter.
Preparation and Storage
Ships on blue ice.
Upon receipt store at 4°C for short term, -20°C for long term .
Upon receipt store at 4°C for short term, -20°C for long term .
Immunocytochemistry (ICC)
(O4 in Differentiated Rat Cortical Stem Cells. Oligodendrocyte Marker O4 was detected in immersion fixed differentiated rat cortical stem cells using 1 ug/mL Human/Mouse/Rat/Chicken O4 antibody (MBS832589) for 3 hours at room temperature.)
Immunocytochemistry (ICC)
(O4 in Differentiated Rat Cortical Stem Cells. Oligodendrocyte Marker O4 was detected in immersion fixed differentiated rat cortical stem cells using 1 ug/mL Human/Mouse/Rat/Chicken O4 antibody (MBS832589) for 3 hours at room temperature.)
Immunocytochemistry (ICC)
(Oligodendrocyte Marker O4 in Rat Cortical Stem Cells. Oligodendrocyte Marker O4 was detected in immersion fixed 7 day differentiated rat cortical stem cells using 1 ug/m Human/Mouse/Rat/Chicken O4 antibody (MBS832589)for 3 hours at room temperature. Cells were stained (red) and counterstained with DAPI (blue).)
Immunocytochemistry (ICC)
(Oligodendrocyte Marker O4 in Rat Cortical Stem Cells. Oligodendrocyte Marker O4 was detected in immersion fixed 7 day differentiated rat cortical stem cells using 1 ug/m Human/Mouse/Rat/Chicken O4 antibody (MBS832589)for 3 hours at room temperature. Cells were stained (red) and counterstained with DAPI (blue).)
Related Product Information for anti-O4 antibody
Mouse monoclonal O4 antibody
Product Categories/Family for anti-O4 antibody
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Product Notes
The O4 (Catalog #AAA832589) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The O4 antibody reacts with Chicken, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's O4 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunohistochemistry (IHC). FC: 2.5 ug/10^6 cells IHC: 110 ug/ml. Researchers should empirically determine the suitability of the O4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "O4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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