Immunohistochemistry (IHC)
(Immunohistochemical analysis of Actin Sarcomeric Muscle staining in human myocardium formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Mouse anti-Human Actin Sarcomeric Muscle Monoclonal Antibody | anti-ACTA1 antibody
Anti-Actin Sarcomeric Muscle Antibody
Gene Names
ACTA1; ACTA; ASMA; CFTD; MPFD; NEM1; NEM2; NEM3; CFTD1; CFTDM
Reactivity
Human
Applications
Immunohistochemistry
Purity
The antibody was purified by immunogen affinity chromatography.
Synonyms
Actin Sarcomeric Muscle; Monoclonal Antibody; Anti-Actin Sarcomeric Muscle Antibody; ACTA; Actin alpha skeletal muscle; Alpha-actin-1; ACTC; Actin alpha cardiac muscle 1; Alpha-cardiac actin; anti-ACTA1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1
Specificity
Recognizes endogenous levels of Actin Sarcomeric Muscle protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.
Applicable Applications for anti-ACTA1 antibody
Immunohistochemistry (IHC)
Application Notes
IHC: 1/100-1/300
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within human Actin Sarcomeric Muscle. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Immunohistochemistry (IHC)
(Immunohistochemical analysis of Actin Sarcomeric Muscle staining in human myocardium formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of Actin Sarcomeric Muscle staining in human myocardium formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunoprecipitation (IP)
(Immunohistochemical analysis of Actin Sarcomeric Muscle staining in human skeletal muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunoprecipitation (IP)
(Immunohistochemical analysis of Actin Sarcomeric Muscle staining in human skeletal muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Related Product Information for anti-ACTA1 antibody
Mouse monoclonal antibody to Actin Sarcomeric Muscle
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
42,051 Da
NCBI Official Full Name
actin, alpha skeletal muscle
NCBI Official Synonym Full Names
actin, alpha 1, skeletal muscle
NCBI Official Symbol
ACTA1
NCBI Official Synonym Symbols
ACTA; ASMA; CFTD; MPFD; NEM1; NEM2; NEM3; CFTD1; CFTDM
NCBI Protein Information
actin, alpha skeletal muscle; nemaline myopathy type 3
UniProt Protein Name
Actin, alpha skeletal muscle
UniProt Gene Name
ACTA1
UniProt Synonym Gene Names
ACTA
UniProt Entry Name
ACTS_HUMAN
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Product Notes
The ACTA1 acta1 (Catalog #AAA8309929) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Actin Sarcomeric Muscle Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Actin Sarcomeric Muscle can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC). IHC: 1/100-1/300. Researchers should empirically determine the suitability of the ACTA1 acta1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Actin Sarcomeric Muscle, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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