Western Blot (WB)
(Western blot analysis of PDE6D expression in HT29 (A), mouse brain (B), mouse kidney (C) whole cell lysates.)
Rabbit PDE6D Polyclonal Antibody | anti-PDE6D antibody
Anti-PDE6D Antibody
IF/ICC: 1:50-1:200
Western Blot (WB)
(Western blot analysis of PDE6D expression in HT29 (A), mouse brain (B), mouse kidney (C) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of PDE6D expression in HT29 (A), mouse brain (B), mouse kidney (C) whole cell lysates.)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(Immunofluorescent analysis of PDE6D staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(Immunofluorescent analysis of PDE6D staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the delta subunit of rod-specific photoreceptor phosphodiesterase (PDE), a key enzyme in the phototransduction cascade. A similar protein in cow functions in solubilizing membrane-bound PDE. In addition to its role in the PDE complex, the encoded protein is thought to bind to prenyl groups of proteins to target them to subcellular organelles called cilia. Mutations in this gene are associated with Joubert syndrome-22. Alternative splicing results in multiple splice variants. [provided by RefSeq, Mar 2014]
Uniprot Description
PDE6D: Acts as a GTP specific dissociation inhibitor (GDI). Increases the affinity of ARL3 for GTP by several orders of magnitude and does so by decreasing the nucleotide dissociation rate. Stabilizes Arl3-GTP by decreasing the nucleotide dissociation. Belongs to the PDE6D/unc-119 family.
Chromosomal Location of Human Ortholog: 2q35-q36
Cellular Component: cytoskeleton; cytoplasmic vesicle membrane; cytoplasmic vesicle; cytosol
Molecular Function: protein binding; 3',5'-cyclic-nucleotide phosphodiesterase activity; Rab GTPase binding; GTPase inhibitor activity
Biological Process: visual perception; metabolic process; organelle organization and biogenesis; negative regulation of catalytic activity; response to stimulus
Disease: Joubert Syndrome 22
Research Articles on PDE6D
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Product Notes
The PDE6D pde6d (Catalog #AAA8307630) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PDE6D Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PDE6D can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC). WB: 1:500-1:2000 IF/ICC: 1:50-1:200. Researchers should empirically determine the suitability of the PDE6D pde6d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDE6D, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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