Rabbit MSRB1 Polyclonal Antibody | anti-MSRB1 antibody

Anti-MSRB1 Antibody

Cat. Num. AAA8307325

• Gene Names: MSRB1; SELR; SELX; SepR; SEPX1; HSPC270
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence, Immunocytochemistry
• Purity: Purified by Immunogen Affinity Chromatography
• Synonyms: MSRB1; Polyclonal Antibody; Anti-MSRB1 Antibody; SEPX1; Methionine-R-sulfoxide reductase B1; MsrB1; Selenoprotein X; SelX; anti-MSRB1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Recognizes endogenous levels of MSRB1 protein.
• Purity/Purification: Purified by Immunogen Affinity Chromatography
• Form/Format: Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.

• Applicable Applications for anti-MSRB1 antibody: Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
• Application Notes: WB: 1:500-1:2000; IF/ICC: 1:50-1:200
• Immunogen: Recombinant fusion protein of human MSRB1. The exact sequence is proprietary.
• Preparation and Storage: Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of MSRB1 expression in HL60 (A), mouse kidney (B), rat brain (C) whole cell lysates.)

Immunofluorescence (IF)/Immunocytochemistry (ICC)

(Immunofluorescent analysis of MSRB1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 488-conjugated secondary antibody (green) in PBS at room temperature in the dark.)

Related Product Information for anti-MSRB1 antibody

Rabbit polyclonal antibody to MSRB1

NCBI and Uniprot Product Information

• NCBI GI #: 182676387
• NCBI GeneID: 51734
• UniProt Accession #: Q9NZV6; Q9JLC3; Q52KJ8
• Molecular Weight: 116
• NCBI Official Full Name: Methionine-R-sulfoxide reductase B1
• NCBI Official Synonym Full Names: methionine sulfoxide reductase B1
• NCBI Official Symbol: MSRB1
• NCBI Official Synonym Symbols: SELR; SELX; SepR; SEPX1; HSPC270
• NCBI Protein Information: methionine-R-sulfoxide reductase B1; selenoprotein R; selenoprotein X, 1
• UniProt Protein Name: Methionine-R-sulfoxide reductase B1
• Protein Family: Methionine-R-sulfoxide reductase
• UniProt Gene Name: MSRB1
• UniProt Synonym Gene Names: SEPX1; MsrB1; SelX
• UniProt Entry Name: MSRB1_HUMAN

NCBI Description

This gene encodes a selenoprotein, which contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenoprotein genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This protein belongs to the methionine sulfoxide reductase (Msr) protein family which includes repair enzymes that reduce oxidized methionine residues in proteins. The protein encoded by this gene is expressed in a variety of adult and fetal tissues and localizes to the cell nucleus and cytosol. [provided by RefSeq, Mar 2012]

Uniprot Description

SEPX1: a selenoprotein, which contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenoprotein genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This protein belongs to the methionine sulfoxide reductase (Msr) protein family which includes repair enzymes that reduce oxidized methionine residues in proteins. The protein encoded by this gene is expressed in a variety of adult and fetal tissues and localizes to the cell nucleus and cytosol. [provided by RefSeq, Mar 2012]

Protein type: Oxidoreductase; EC 1.8.4.-

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: cytoplasm; nucleus; actin cytoskeleton

Molecular Function: zinc ion binding; peptide-methionine (R)-S-oxide reductase activity; actin binding

Biological Process: protein repair; actin filament polymerization; innate immune response; response to oxidative stress