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Western Blot (WB) (Western blot analysis of MED20 expression in DU145 (A), mouse brain (B) whole cell lysates.)

Rabbit MED20 Polyclonal Antibody | anti-MED20 antibody

Anti-MED20 Antibody

Gene Names
MED20; TRFP; PRO0213
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Purified by Immunogen Affinity Chromatography
Synonyms
MED20; Polyclonal Antibody; Anti-MED20 Antibody; TRFP; Mediator of RNA polymerase II transcription subunit 20; Mediator complex subunit 20; TRF-proximal protein homolog; hTRFP; anti-MED20 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of MED20 protein.
Purity/Purification
Purified by Immunogen Affinity Chromatography
Form/Format
Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.
Applicable Applications for anti-MED20 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
Recombinant fusion protein of human MED20. The exact sequence is proprietary.
Preparation and Storage
Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of MED20 expression in DU145 (A), mouse brain (B) whole cell lysates.)

Western Blot (WB) (Western blot analysis of MED20 expression in DU145 (A), mouse brain (B) whole cell lysates.)
Related Product Information for anti-MED20 antibody
Rabbit polyclonal antibody to MED20

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
13,540 Da
NCBI Official Full Name
mediator of RNA polymerase II transcription subunit 20
NCBI Official Synonym Full Names
mediator complex subunit 20
NCBI Official Symbol
MED20
NCBI Official Synonym Symbols
TRFP; PRO0213
NCBI Protein Information
mediator of RNA polymerase II transcription subunit 20; TRF-proximal protein homolog; Trf (TATA binding protein-related factor)-proximal homolog; hTRFP; mediator complex subunit 20mediator complex subunit 20mediator complex subunit 20Trf (TATA binding pro
UniProt Protein Name
Mediator of RNA polymerase II transcription subunit 20
UniProt Gene Name
MED20
UniProt Synonym Gene Names
TRFP; hTRFP
UniProt Entry Name
MED20_HUMAN

NCBI Description

MED20 is a subunit of the Mediator complex, a multiprotein coactivator of RNA transcription that interacts with DNA-bound transcriptional activators, RNA polymerase II (see MIM 180660), and general initiation factors (Sato et al., 2003 [PubMed 14576168]).[supplied by OMIM, Aug 2009]

Uniprot Description

TRFP: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Belongs to the Mediator complex subunit 20 family.

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: nucleoplasm; Srb-mediator complex

Molecular Function: protein binding; DNA-directed RNA polymerase activity

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; gene expression

Similar Products

Product Notes

The MED20 med20 (Catalog #AAA8307245) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-MED20 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MED20 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the MED20 med20 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MED20, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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