EPHA1 recombinant protein

Recombinant Human EPHA1 Protein

Cat. Num. AAA8304978

• Gene Names: EPHA1; EPH; EPHT; EPHT1
• Applications: ELISA, Western Blot, SDS-Page
• Purity: >95% as determined by reducing SDS-PAGE
• Synonyms: EPHA1; Recombinant Human EPHA1 Protein; EPH; EPHT; EPHT1; Ephrin type-A receptor 1; hEpha1; EPH tyrosine kinase; EPH tyrosine kinase 1; Erythropoietin-producing hepatoma receptor; Tyrosine-protein kinase receptor EPH; EPHA1 recombinant protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE
• Form/Format: Lyophilized from a 0.2 mum filtered solution of PBS, pH7.4.
• Sequence: KEVTLMDTSK AQGELGWLLD PPKDGWSEQQ QILNGTPLYM YQDCPMQGRR DTDHWLRSNW IYRGEEASRV HVELQFTVRD CKSFPGGAGP LGCKETFNLL YMESDQDVGI QLRRPLFQKV TTVAADQSFT IRDLASGSVK LNVERCSLGR LTRRGLYLAF HNPGACVALV SVRVFYQRCP ETLNGLAQFP DTLPGPAGLV EVAGTCLPHA RASPRPSGAP RMHCSPDGEW LVPVGRCHCE PGYEEGGSGE ACVACPSGSY RMDMDTPHCL TCPQQSTAES EGATICTCES GHYRAPGEGP QVACTGPPSA PRNLSFSASG TQLSLRWEPP ADTGGRQDVR YSVRCSQCQG TAQDGGPCQP CGVGVHFSPG ARGLTTPAVH VNGLEPYANY TFNVEAQNGV SGLGSSGHAS TSVSISMGHA ESLSGLSLRL VKKEPRQLEL TWAGSRPRSP GANLTYELHV LNQDEERYQM VLEPRVLLTE LQPDTTYIVR VRMLTPLGPG PFSPDHEFRT SPPVSRGLTG GEHHHHHH
• Sequence Length: 976

• Applicable Applications for EPHA1 recombinant protein: ELISA (EIA), Western Blot (WB), SDS-PAGE
• Endotoxin: Less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.
• Preparation and Storage: Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks.; Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.

Related Product Information for EPHA1 recombinant protein

Recombinant Human EPHA1 Protein is produced by mammalian expression system and the target gene encoding Lys26-Glu547 is expressed with a 6His tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 221316650
• NCBI GeneID: 2041
• NCBI Accession #: NP_005223.4
• NCBI GenBank Nucleotide #: NP_005223.4
• UniProt Accession #: P21709
• NCBI Official Full Name: ephrin type-A receptor 1
• NCBI Official Synonym Full Names: EPH receptor A1
• NCBI Official Symbol: EPHA1
• NCBI Official Synonym Symbols: EPH; EPHT; EPHT1
• NCBI Protein Information: ephrin type-A receptor 1
• UniProt Protein Name: Ephrin type-A receptor 1
• Protein Family: Ephrin type-A receptor
• UniProt Gene Name: EPHA1
• UniProt Synonym Gene Names: EPH; EPHT; EPHT1; hEpha1
• UniProt Entry Name: EPHA1_HUMAN

NCBI Description

This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene is expressed in some human cancer cell lines and has been implicated in carcinogenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

EphA1: a receptor tyrosine kinase. Receptor for members of the ephrin-A family. Binds with a low affinity to ephrin-A1. The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels. Misexpressed in several cancers, including upregulation in head and neck cancer, and downregulation in invasive breast cancer cell lines and glioblastoma

Protein type: Membrane protein, integral; EC 2.7.10.1; Protein kinase, TK; Protein kinase, tyrosine (receptor); Kinase, protein; TK group; Eph family

Chromosomal Location of Human Ortholog: 7q34

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: transmembrane-ephrin receptor activity; protein kinase binding; ATP binding; protein kinase activity

Biological Process: axon guidance; positive regulation of angiogenesis; peptidyl-tyrosine phosphorylation; cell surface receptor linked signal transduction; somatic stem cell maintenance; protein amino acid autophosphorylation; positive regulation of cell proliferation; positive regulation of stress fiber formation; ephrin receptor signaling pathway; negative regulation of protein kinase activity; angiogenesis; positive regulation of cell-matrix adhesion; negative regulation of cell migration; positive regulation of cell migration