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Western Blot (WB) (Western Blot analysis of ectracts from HepG2 cells)

Rabbit anti-Human c-Met Polyclonal Antibody | anti-MET antibody

c-Met, phosphorylated (Tyr1349) (Hepatocyte Growth Factor Receptor, Scatter Factor Receptor, HGFR, SFR)

Gene Names
MET; c-Met
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Synonyms
c-Met; Polyclonal Antibody; phosphorylated (Tyr1349) (Hepatocyte Growth Factor Receptor; Scatter Factor Receptor; HGFR; SFR); Anti -c-Met; anti-MET antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Detects endogenous levels of Met only when phosphorylated at tyrosine 1349.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide and 50% glycerol.
Applicable Applications for anti-MET antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Dilution: Western Blot: 1:500-1:1000
Immunogen
Synthesized phosphopeptide derived from human Met around the phosphorylation site of tyrosine 1349 (E-H-YP-V-H)
Preparation and Storage
For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot analysis of ectracts from HepG2 cells)

Western Blot (WB) (Western Blot analysis of ectracts from HepG2 cells)
Product Categories/Family for anti-MET antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
154,637 Da
NCBI Official Full Name
c-Met
NCBI Official Symbol
MET
NCBI Official Synonym Symbols
c-Met
NCBI Protein Information
hepatocyte growth factor receptor; SF receptor; HGF receptor; HGF/SF receptor; proto-oncogene c-Met; scatter factor receptor; tyrosine-protein kinase Met; met proto-oncogene tyrosine kinase
UniProt Protein Name
Hepatocyte growth factor receptor
Protein Family
UniProt Gene Name
MET
UniProt Entry Name
MET_CANFA

Uniprot Description

Function: Receptor for hepatocyte growth factor and scatter factor. Has a tyrosine-protein kinase activity. Functions in cell proliferation, scattering, morphogenesis and survival

Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Subunit structure: Heterodimer formed of an alpha chain (50 kDa) and a beta chain (145 kDa) which are disulfide linked. Binds PLXNB1 and GRB2. Interacts with INPP5D/SHIP1. When phosphorylated at Tyr-1357, interacts with INPPL1/SHIP2. Interacts with RANBP9 and RANBP10, as well as SPSB1, SPSB2, SPSB3 and SPSB4. SPSB1 binding occurs in the presence and in the absence of HGF, however HGF treatment has a positive effect on this interaction. Interacts with MUC20; prevents interaction with GRB2 and suppresses hepatocyte growth factor-induced cell proliferation. Interacts with GRB10

Subcellular location: Membrane; Single-pass type I membrane protein

Domain: The kinase domain is involved in SPSB1 binding

Post-translational modification: Dephosphorylated by PTPRJ at Tyr-1350 and Tyr-1366

Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family.Contains 3 IPT/TIG domains.Contains 1 protein kinase domain.Contains 1 Sema domain.

Research Articles on MET

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Product Notes

The MET met (Catalog #AAA630599) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The c-Met, phosphorylated (Tyr1349) (Hepatocyte Growth Factor Receptor, Scatter Factor Receptor, HGFR, SFR) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's c-Met can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in ELISA, Western Blot and Immunohistochemistry. Dilution: Western Blot: 1:500-1:1000. Researchers should empirically determine the suitability of the MET met for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "c-Met, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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